Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients/Experiment 1

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Reviewed Marked as Reviewed by Chloe on 2021/08/10

Curated date: 2021/06/17

Curator: Claregrieve1

Revision editor(s): WikiWorks, Chloe, Claregrieve1, Peace Sandy

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Nasopharynx Nasenrachenraum,Epipharynx,Nasal part of pharynx,Pars nasalis pharyngis,Rhinopharynx,Nasopharynx,nasopharynx
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
COVID-19 2019 novel coronavirus,2019 novel coronavirus infection,2019-nCoV,2019-nCoV infection,beta-CoV,beta-CoVs,betacoronavirus,coronavirus disease 2019,SARS-coronavirus 2,SARS-CoV-2,severe acute respiratory syndrome coronavirus 2,severe acute respiratory syndrome coronavirus 2 infectious disease,β-coronavirus,β-CoV,β-CoVs,COVID-19,cOVID-19
Group 0 name Corresponds to the control (unexposed) group for case-control studies
COVID-19 negative controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
COVID-19 positive patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients who are COVID-19 positive by RT-PCR
Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
9

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Pielou Quantifies how equal the community is numerically
unchanged
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged
Richness Number of species
unchanged

Signature 1

Reviewed Marked as Reviewed by Chloe on 2021/08/11

Curated date: 2021/06/17

Curator: Claregrieve1

Revision editor(s): WikiWorks, Chloe, Claregrieve1, Merit

Source: Supplementary Tables 5 & 6

Description: Differential abundance of nasopharyngeal bacteria between COVID-19-positive and COVID-19-negative samples

Abundance in Group 1: decreased abundance in COVID-19 positive patients

NCBI Quality ControlLinks
Alloprevotella
Anaerococcus
Bacteroidota
Blastococcus
Butyricicoccus
Campylobacter
Capnocytophaga
Chryseobacterium
Finegoldia
Flavobacterium
Gemella
Kocuria
Micrococcus
Moraxella
Peptoniphilus
Porphyromonas
Prevotella
Rothia
Selenomonas
Veillonella
Vibrio
unclassified Bacilli

Revision editor(s): WikiWorks, Chloe, Claregrieve1, Merit

Signature 2

Reviewed Marked as Reviewed by Chloe on 2021/08/10

Curated date: 2021/06/17

Curator: Claregrieve1

Revision editor(s): Claregrieve1

Source: Supplementary Tables 5 & 6

Description: Differential abundance of nasopharyngeal bacteria between COVID-19-positive and COVID-19-negative samples

Abundance in Group 1: increased abundance in COVID-19 positive patients

NCBI Quality ControlLinks
Tumebacillus
Gulbenkiania

Revision editor(s): Claregrieve1