Characteristics of the intestinal flora in patients with peripheral neuropathy associated with type 2 diabetes

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/22

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

32938282

DOI Digital object identifier for electronic documents.

10.1177/0300060520936806

URI

https://pubmed.ncbi.nlm.nih.gov/32938282/

Authors

Wang Y, Ye X, Ding D, Lu Y

Journal

The Journal of international medical research

Year

2020

Keywords:

Diabetic peripheral neuropathy, bile acids, blood chemistry, gastrointestinal microbiota, insulin resistance, microbial diversity, richness, type 2 diabetes

OBJECTIVE: To study the characteristics of the intestinal flora in patients with diabetic peripheral neuropathy (DPN) and analyze the association between the intestinal flora and clinical indicators. METHODS: We classified 80 subjects into three groups: patients with DPN (n = 45), patients type 2 diabetes without DPN (n = 21), and healthy controls (n = 14). The intestinal flora composition was compared among the three groups, and the correlation between the intestinal flora and clinical indicators was analyzed. RESULTS: At the phylum level, the richness of Firmicutes and Actinobacteria was elevated in the DN group, and that of Bacteroidetes was decreased. At the genus level, the richness of Bacteroides and Faecalibacterium was significantly decreased in the DPN group, whereas that of Escherichia-Shigella, Lachnoclostridium, Blautia, Megasphaera, and Ruminococcus torques group was increased. The homeostasis model assessment insulin resistance index was positively correlated with Megasphaera richness. Glycine ursodeoxycholic acid was positively correlated with Ruminococcus gnavus group and Phascolarctobacterium richness. Tauroursodeoxycholic acid was positively correlated with Ruminococcus gnavus group and Parabacteroides richness. CONCLUSION: There was obvious intestinal microbiota disorder in patients with DPN, which may be related to insulin resistance. These changes may have important roles in the development of DPN.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/22

Curated date: 2021/06/21

Curator: Madhubani Dey

Revision editor(s): WikiWorks, Rimsha, Madhubani Dey, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Diabetic neuropathy Amyotrophies, Diabetic,Amyotrophy, Diabetic,Asymmetric Polyneuropathy, Diabetic,Diabetic Amyotrophies,Diabetic Amyotrophy,Diabetic Asymmetric Polyneuropathies,Diabetic Asymmetric Polyneuropathy,Diabetic Autonomic Neuropathies,Diabetic Autonomic Neuropathy,Diabetic Mononeuropathies,Diabetic Mononeuropathy,Diabetic Mononeuropathy Simplex,Diabetic Mononeuropathy Simplices,diabetic neuropathies,diabetic neuropathy,Painful Diabetic Neuropathies,Painful Diabetic Neuropathy,Diabetic neuropathy

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Individuals diagnosed with peripheral neuropathy associated with type 2 diabetes

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Individuals diagnosed with peripheral neuropathy associated with type 2 diabetes; The criteria for DPN were as follows: spontaneous limb pain, symmetrical or unilateral limb numbness, sensation of dullness and body tension, muscle weakness, weakened or absent tendon reflexes, and significant decreases in sensory and motor nerve conduction velocities as revealed via electromyography indicating the positive involvement of two or more nerves and normal arterial pulses in the foot and back.

Group 0 sample size Number of subjects in the control (unexposed) group: 14

Group 1 sample size Number of subjects in the case (exposed) group: 45

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2021/06/24

Curator: Madhubani Dey

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Source: Figure 5a, 5b

Description: Differential abundance of bacterial communities in individuals with diabetic peripheral neuropathy compared with healthy controls

Abundance in Group 1: increased abundance in Individuals diagnosed with peripheral neuropathy associated with type 2 diabetes

NCBI	Quality Control	Links
Actinomycetota
Blautia
Escherichia
Escherichia/Shigella sp.
Bacillota
Lachnoclostridium
Megasphaera
[Ruminococcus] torques

Revision editor(s): Madhubani Dey, Claregrieve1

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/22

Curated date: 2021/06/24

Curator: Madhubani Dey

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Source: Figure 5a, 5b

Description: Differential abundance of bacterial communities in individuals with diabetic peripheral neuropathy compared with healthy controls

Abundance in Group 1: decreased abundance in Individuals diagnosed with peripheral neuropathy associated with type 2 diabetes

NCBI	Quality Control	Links
Bacteroides
Bacteroidota
Faecalibacterium

Revision editor(s): Madhubani Dey, Claregrieve1

Experiment 2

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Curated date: 2021/06/24

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Individuals diagnosed with type 2 diabetes Mellitus

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with peripheral neuropathy associated with type 2 diabetes; the criteria for DPN were as follows: spontaneous limb pain, symmetrical or unilateral limb numbness, sensation of dullness and body tension, muscle weakness, weakened or absent tendon reflexes, and significant decreases in sensory and motor nerve conduction velocities as revealed via electromyography indicating the positive involvement of two or more nerves and normal arterial pulses in the foot and back.

Group 0 sample size Number of subjects in the control (unexposed) group: 21

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Experiment 3

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/22

Curated date: 2021/06/24

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Type II diabetes mellitus adult onset diabetes,Adult-Onset Diabetes,adult-onset diabetes,Adult-Onset Diabetes Mellitus,diabetes mellitis type 2,diabetes mellitis type II,DIABETES MELLITUS TYPE 02,diabetes mellitus type 2,Diabetes Mellitus, Adult Onset,Diabetes Mellitus, Adult-Onset,Diabetes Mellitus, Ketosis Resistant,Diabetes Mellitus, Ketosis-Resistant,Diabetes Mellitus, Maturity Onset,Diabetes Mellitus, Maturity-Onset,Diabetes Mellitus, Non Insulin Dependent,Diabetes Mellitus, Non-Insulin-Dependent,Diabetes Mellitus, Noninsulin Dependent,diabetes mellitus, noninsulin-dependent,Diabetes Mellitus, Slow Onset,Diabetes Mellitus, Slow-Onset,Diabetes Mellitus, Stable,Diabetes Mellitus, Type 2,diabetes mellitus, type 2,diabetes mellitus, type 2, protection against,Diabetes Mellitus, Type II,Diabetes, Type 2,diabetes, type 2,insulin resistance, susceptibility to,Ketosis-Resistant Diabetes Mellitus,Maturity Onset Diabetes Mellitus,maturity-onset diabetes,Maturity-Onset Diabetes Mellitus,MODY,NIDDM,Non-Insulin Dependent Diabetes,non-insulin dependent diabetes,Non-Insulin Dependent Diabetes Mellitus,non-insulin dependent diabetes mellitus,non-insulin-dependent diabetes mellitus,noninsulin dependent diabetes,noninsulin-dependent diabetes mellitus,Slow-Onset Diabetes Mellitus,Stable Diabetes Mellitus,T2DM - Type 2 Diabetes mellitus,T2DM - type 2 diabetes mellitus,Type 2 Diabetes,type 2 diabetes,Type 2 Diabetes Mellitus,type 2 diabetes mellitus,Type 2 Diabetes Mellitus Non-Insulin Dependent,type 2 diabetes mellitus non-insulin dependent,Type II Diabetes,type II diabetes,type II diabetes mellitus,Type II diabetes mellitus

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Individuals diagnosed with Type 2 Diabetes Mellitus

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Individuals diagnosed with Type 2 Diabetes Mellitus; The diagnoses of type 2 diabetes mellitus were established according to the diagnostic criteria of the American Diabetes Association