Characteristics of the intestinal flora in patients with peripheral neuropathy associated with type 2 diabetes/Experiment 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/22

Curated date: 2021/06/24

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Diabetic neuropathy Amyotrophies, Diabetic,Amyotrophy, Diabetic,Asymmetric Polyneuropathy, Diabetic,Diabetic Amyotrophies,Diabetic Amyotrophy,Diabetic Asymmetric Polyneuropathies,Diabetic Asymmetric Polyneuropathy,Diabetic Autonomic Neuropathies,Diabetic Autonomic Neuropathy,Diabetic Mononeuropathies,Diabetic Mononeuropathy,Diabetic Mononeuropathy Simplex,Diabetic Mononeuropathy Simplices,diabetic neuropathies,diabetic neuropathy,Painful Diabetic Neuropathies,Painful Diabetic Neuropathy,Diabetic neuropathy

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Individuals diagnosed with type 2 diabetes Mellitus

Group 1 name Corresponds to the case (exposed) group for case-control studies

Individuals diagnosed with peripheral neuropathy associated with type 2 diabetes

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Patients with peripheral neuropathy associated with type 2 diabetes; the criteria for DPN were as follows: spontaneous limb pain, symmetrical or unilateral limb numbness, sensation of dullness and body tension, muscle weakness, weakened or absent tendon reflexes, and significant decreases in sensory and motor nerve conduction velocities as revealed via electromyography indicating the positive involvement of two or more nerves and normal arterial pulses in the foot and back.

Group 0 sample size Number of subjects in the control (unexposed) group

21

Group 1 sample size Number of subjects in the case (exposed) group

45

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

3 months

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Statistical Analysis

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

increased

Chao1 Abundance-based estimator of species richness

increased

Simpson Estimator of species richness and species evenness: more weight on species evenness

unchanged