The Endobiota Study: Comparison of Vaginal, Cervical and Gut Microbiota Between Women with Stage 3/4 Endometriosis and Healthy Controls

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

study design

prospective cohort

Citation

PMID PubMed identifier for scientific articles.

30778155

DOI Digital object identifier for electronic documents.

10.1038/s41598-019-39700-6

URI

https://pubmed.ncbi.nlm.nih.gov/30778155/

Authors

Ata B, Yildiz S, Turkgeldi E, Brocal VP, Dinleyici EC, Moya A, Urman B

Journal

Scientific reports

Year

2019

Dysbiosis in the genital tract or gut microbiome can be associated with endometriosis. We sampled vaginal, cervical and gut microbiota from 14 women with histology proven stage 3/4 endometriosis and 14 healthy controls. The V3 and V4 regions of the 16S rRNA gene were amplified following the 16S Metagenomic Sequencing Library Preparation. Despite overall similar vaginal, cervical and intestinal microbiota composition between stage 3/4 endometriosis group and controls, we observed differences at genus level. The complete absence of Atopobium in the vaginal and cervical microbiota of the stage 3/4 endometriosis group was noteworthy. In the cervical microbiota, Gardnerella, Streptococcus, Escherichia, Shigella, and Ureoplasma, all of which contain potentially pathogenic species, were increased in stage 3/4 endometriosis. More women in the stage 3/4 endometriosis group had Shigella/Escherichia dominant stool microbiome. Further studies can clarify whether the association is causal, and whether dysbiosis leads to endometriosis or endometriosis leads to dysbiosis.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2021/06/24

Curator: Samara.Khan

Revision editor(s): Samara.Khan, WikiWorks, Peace Sandy

Subjects

Location of subjects: Turkey

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Vaginal fluid , Feces , Endocervix Vaginal discharge,Vaginal secretion,Vaginal fluid,vaginal fluid,Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces,Endocervix,endocervix

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Stage 3/4 endometriosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women with histology-proven stage 3/4 endometriosis

Group 0 sample size Number of subjects in the control (unexposed) group: 14

Group 1 sample size Number of subjects in the case (exposed) group: 14

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Participants were excluded if they had taken antibiotics within the past 8 weeks

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2021/06/24

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Peace Sandy

Source: Table 1

Description: Diferences between microbiota in women with endometriosis and healthy controls.

Abundance in Group 1: increased abundance in Stage 3/4 endometriosis patients

NCBI	Quality Control	Links
Gardnerella
Escherichia/Shigella sp.
Alloprevotella
Streptococcus
Ureaplasma

Revision editor(s): Samara.Khan, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-5

Curated date: 2024/02/05

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Source: Table 1

Description: Diferences between microbiota in women with endometriosis and healthy controls.

Abundance in Group 1: decreased abundance in Stage 3/4 endometriosis patients

NCBI	Quality Control	Links
Sneathia
Prevotella
Dialister
Megasphaera
Gardnerella
Barnesiella

Revision editor(s): Peace Sandy