Obesity Alters the Microbial Community Profile in Korean Adolescents
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Hu HJ, Park SG, Jang HB, Choi MK, Choi MG, Park KH, Kang JH, Park SI, Lee HJ, Cho SH
Journal
PloS one
Year
2015
Obesity is an increasing public health concern worldwide. According to the latest Organization for Economic Co-operation and Development (OECD) report (2014), the incidence of child obesity in Korea has exceeded the OECD average. To better understand and control this condition, the present study examined the composition of the gut microbial community in normal and obese adolescents. Fecal samples were collected from 67 obese (body mass index [BMI] ≥ 30 kg/m2, or ≥ 99th BMI percentile) and 67 normal (BMI < 25 kg/m2 or < 85th BMI percentile) Korean adolescents aged 13-16 years and subjected to 16S rRNA gene sequencing. Analysis of bacterial composition according to taxonomic rank (genus, family, and phylum) revealed marked differences in the Bacteroides and Prevotella populations in normal and obese samples (p < 0.005) at the genus and family levels; however, there was no difference in the Firmicutes-to-Bacteroidetes (F/B) ratio between normal and obese adolescents samples at the phylum level (F/B normal = 0.50 ± 0.53; F/B obese = 0.56 ± 0.86; p = 0.384). Statistical analysis revealed a significant association between the compositions of several bacterial taxa and child obesity. Among these, Bacteroides and Prevotella showed the most significant association with BMI (p < 0.0001 and 0.0001, respectively). We also found that the composition of Bacteroides was negatively associated with triglycerides (TG), total cholesterol, and high-sensitive C-reactive protein (hs-crp) (p = 0.0049, 0.0023, and 0.0038, respectively) levels, whereas that of Prevotella was positively associated with TG and hs-crp levels (p = 0.0394 and 0.0150, respectively). We then applied the association rule mining algorithm to generate "rules" to identify the association between the populations of multiple bacterial taxa and obesity; these rules were able to discriminate obese from normal states. Therefore, the present study describes a systemic approach to identify the association between bacterial populations in the gut and childhood obesity.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- South Korea
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- control adolescent
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese adolescent
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- body mass index BMI >= 30 kg/m2, or >= 99th BMI percentile
- Group 0 sample size Number of subjects in the control (unexposed) group
- 67
- Group 1 sample size Number of subjects in the case (exposed) group
- 67
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 1 month
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2 and Table 3
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: increased abundance in obese adolescent
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae | ||
| Sutterellaceae | ||
| Veillonellaceae | ||
| Prevotella |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2 and Table 3
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: decreased abundance in obese adolescent
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroidaceae | ||
| Rikenellaceae | ||
| Oscillospiraceae | ||
| Bacteroides | ||
| Alistipes | ||
| Faecalibacterium | ||
| Oscillibacter |
Revision editor(s): WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Fatima on 2021/06/29
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low BMI zscores
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high BMI zscore
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
- Statistical test
- Spearman Correlation
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: increased abundance in high BMI zscore
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: decreased abundance in high BMI zscore
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Alistipes |
Revision editor(s): WikiWorks
Experiment 3
Reviewed Marked as Reviewed by Fatima on 2021/06/29
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low triglycerides
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high triglycerides
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: increased abundance in high triglycerides
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: decreased abundance in high triglycerides
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides |
Revision editor(s): WikiWorks
Experiment 4
Reviewed Marked as Reviewed by Fatima on 2021/07/16
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low total cholesterol
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high total cholesterol
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: decreased abundance in high total cholesterol
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides |
Revision editor(s): WikiWorks
Experiment 5
Reviewed Marked as Reviewed by Fatima on 2021/07/16
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low HDL-C
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high HDL-C
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: increased abundance in high HDL-C
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides |
Revision editor(s): WikiWorks
Experiment 6
Reviewed Marked as Reviewed by Fatima on 2021/07/16
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low sensetive C reactive protein
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high senstive C reactive protein
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: increased abundance in high senstive C reactive protein
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotella |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 4
Description: Relative microbial abundance in obese Korean adolescents (13-16 year old)
Abundance in Group 1: decreased abundance in high senstive C reactive protein
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Oscillibacter |
Revision editor(s): WikiWorks
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