The impact of NBUVB on microbial community profiling in the lesional skin of vitiligo subjects

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

31917273

DOI Digital object identifier for electronic documents.

10.1016/j.micpath.2019.103943

URI

https://pubmed.ncbi.nlm.nih.gov/31917273/

Authors

Yuan X, Wang L, Meng D, Wu L, Wang X, Zhang D, Luo Z, Pang Y, Liu G

Journal

Microbial pathogenesis

Year

2020

Keywords:

16S rRNA, Bacterial diversity, Cutaneous microbiome, Vitiligo

BACKGROUND: The impact of NBUVB on the cutaneous microbiota of vitiligo patients remains to be fully elucidated. METHODS: To characterize the cutaneous microbiota in vitiligo patients, cutaneous samples from 60 patients with vitiligo and after NBUVB irradiation were profiled using the Illumina MiSeq platform. Alpha diversity estimations revealed higher microbiota diversity in samples from patients with lesional skin. Beta diversity (Principal Component Analysis (PCA)) analysis showed that the bacterial community structure segregated differently between different groups. RESULTS: There was a statistically significant increase in the Sobs, ACE, and Chao indices in the NB group compared with NF group, as determined by t-test. The alpha diversity have no significant difference between NF and DB group. At the phylum level, Firmicutes, Proteobacteria and Actinobacteria were the most predominant phyla. Propionibacterium and Pseudomonas were the most predominant genera in each group. In addition, Staphylococcus, Bacillus and Prevotella were enriched in DF group compared to DB group. Propionibacterium was enriched in DB group compared to DF group. CONCLUSIONS: Our studies indicate differences in microbial community dynamics of the lesional and non-lesional sites of vitiligo subjects, with greater diversity and higher association between microbial communities of the unaffected site. And NBUVB irradiation might eliminate these differences.

Experiment 1

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Curated date: 2021/07/13

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Vitiligo VAMAS6,vitiligo,vitiligo-associated multiple autoimmune disease susceptibility 6,Vitiligo

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Vitiligo patients before narrowband UV (NBUVB) light irradiation in normal skin (NF group)
Group 1 name Corresponds to the case (exposed) group for case-control studies: Vitiligo patients after NBUVB irradiation on normal skin (NB group)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Vitiligo patients after NBUVB irradiation on normal skin (NB group); Vitiligo patients recruited from Weifang Medical University Hospital, Shandong, China, from February 2018 to January 2019.
Group 0 sample size Number of subjects in the control (unexposed) group: 15
Group 1 sample size Number of subjects in the case (exposed) group: 15
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged
Chao1 Abundance-based estimator of species richness: increased
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2023/04/24

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Source: FIG. 3

Description: Relative abundance of predominant bacteria at phylum and genus level in the NF and NB groups.

Abundance in Group 1: increased abundance in Vitiligo patients after NBUVB irradiation on normal skin (NB group)

NCBI	Quality Control	Links
Cyanobacteriota
Bacillota
Bacillus
Corynebacterium

Revision editor(s): Atrayees

Experiment 2

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Curated date: 2021/07/13

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Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Normal skin of Vitiligo patients before narrowband UV (NBUVB) light irradiation (NF group)
Group 1 name Corresponds to the case (exposed) group for case-control studies: Lesional skin of Vitiligo patients after NBUVB irradiation (DB group)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Lesional skin of Vitiligo patients after NBUVB irradiation (DB group); Vitiligo patients recruited from Weifang Medical University Hospital, Shandong, China, from February 2018 to January 2019.

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged
Chao1 Abundance-based estimator of species richness: unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Experiment 3

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Curated date: 2021/07/13

Curator: Madhubani Dey

Revision editor(s): Madhubani Dey, WikiWorks, Aiyshaaaa

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Lesional skin of Vitiligo patients before irradiation (DF group)

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged
Chao1 Abundance-based estimator of species richness: unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged