The impact of NBUVB on microbial community profiling in the lesional skin of vitiligo subjects/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by Folakunmi on 2024-2-2

Curated date: 2021/07/13

Curator: Madhubani Dey

Revision editor(s): WikiWorks, Madhubani Dey, Folakunmi

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Skin of body Entire integument,Entire skin,Integument,Integumental organ,Pelt,Skin,Skin organ,Skin of body,skin of body
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Vitiligo VAMAS6,vitiligo,vitiligo-associated multiple autoimmune disease susceptibility 6,Vitiligo
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Normal skin of Vitiligo patients before narrowband UV (NBUVB) light irradiation (NF group)
Group 1 name Corresponds to the case (exposed) group for case-control studies
Lesional skin of Vitiligo patients after NBUVB irradiation (DB group)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Lesional skin of Vitiligo patients after NBUVB irradiation (DB group); Vitiligo patients recruited from Weifang Medical University Hospital, Shandong, China, from February 2018 to January 2019.
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
15
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Folakunmi on 2024-2-2

Curated date: 2024/02/02

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: within results text (Alterations in the composition of cutaneous microbiota associated with vitiligo at the phylum level, lines 9-11)

Description: Relative abundance of predominant bacteria at phylum and genus level in the NF and DB groups.

Abundance in Group 1: increased abundance in Lesional skin of Vitiligo patients after NBUVB irradiation (DB group)

NCBI Quality ControlLinks
Bacteroidia
Pseudomonadota

Revision editor(s): Folakunmi

Signature 2

Reviewed Marked as Reviewed by Folakunmi on 2024-2-2

Curated date: 2024/02/02

Curator: Folakunmi

Revision editor(s): Folakunmi

Source: within results text (Alterations in the composition of cutaneous microbiota associated with vitiligo at the phylum level, lines 9-11)

Description: Relative abundance of predominant bacteria at phylum and genus level in the NF and DB groups.

Abundance in Group 1: decreased abundance in Lesional skin of Vitiligo patients after NBUVB irradiation (DB group)

NCBI Quality ControlLinks
Bacillota
Actinomycetota

Revision editor(s): Folakunmi