Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients/Experiment 3

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Reviewed Marked as Reviewed by Chloe on 2022/03/17

Curated date: 2021/07/19

Curator: Kwekuamoo

Revision editor(s): LGeistlinger, Kwekuamoo, WikiWorks

Subjects

Location of subjects

Netherlands

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces , Mucosa of small intestine Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces,Mucosa of organ of small bowel,Mucosa of organ of small intestine,Mucosa of small bowel,Mucous membrane of small bowel,Mucous membrane of small intestine,Organ mucosa of small bowel,Organ mucosa of small intestine,Small bowel mucosa,Small bowel mucosa of organ,Small bowel mucous membrane,Small bowel organ mucosa,Small intestinal mucosa,Small intestine mucosa,Small intestine mucosa of organ,Small intestine mucous membrane,Small intestine organ mucosa,Tunica mucosa (intestinum tenue),Tunica mucosa intestini tenuis,Mucosa of small intestine,mucosa of small intestine

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Irritable bowel syndrome [X]Psychogenic IBS,Adaptive colitis,Colitides, Mucous,Colitis, Mucous,Colon spasm,Colon, Irritable,Functional bowel disease,IBD,IBS,IBS - Irritable bowel syndrome,IC - Irritable colon,Irritable bowel,Irritable bowel - IBS,irritable bowel syndrome,Irritable Bowel Syndromes,Irritable Colon,irritable colon,Irritable colon (disorder),Irritable colon - Irritable bowel syndrome,Irritable colon syndrome,Membranous colitis,Mucous Colitides,Mucous colitis,mucus colitis,Nervous colitis,Psychogenic IBS,psychogenic IBS,Spastic colitis,Spastic colon,spastic colon,Syndrome, Irritable Bowel,Syndromes, Irritable Bowel,Irritable bowel syndrome

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Healthy Control

Group 1 name Corresponds to the case (exposed) group for case-control studies

IBS-A

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

IBS patients included in this study fulfilled the Rome II criteria for IBS and were categorized as alternating IBS subgroup (IBS-A)

Group 0 sample size Number of subjects in the control (unexposed) group

26

Group 1 sample size Number of subjects in the case (exposed) group

16

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

Subjects taking medication known to influence bacterial composition and gastrointestinal motility, especially antimicrobial medications and/or probiotics were excluded from the study.

Lab analysis

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

RT-qPCR

Statistical Analysis

Statistical test

T-Test

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes