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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/08/08
Curator: Samara.Khan
Revision editor(s): Samara.Khan, Chloe, WikiWorks, Victoria
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy mice
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mice who were injected with endometrial tissues
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Researchers induced endometriosis models by intraperitoneal injection of endometrial tissues to mimic endometriosis formation in humans.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 4
- Group 1 sample size Number of subjects in the case (exposed) group
- 4
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- N/A
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Source: Figure 4
Description: Researchers used t-tests to calculate the differences in microbiota at the taxon level between the control and experimental groups.
The following bacterial taxa were more abundant in mice with simulated endometriosis than in mice without endometriosis.
Abundance in Group 1: increased abundance in Mice who were injected with endometrial tissues
NCBI | Quality Control | Links |
---|---|---|
Actinomycetota | ||
Alcaligenaceae | ||
Betaproteobacteria | ||
Bifidobacteriaceae | ||
Bifidobacteriales | ||
Bifidobacterium | ||
Burkholderiales | ||
Bacillota | ||
Parasutterella |
Revision editor(s): Fatima, Samara.Khan
Signature 2
Source: Figure 4
Description: Researchers used the LEfse method to determine the predominant microbiota in each sample. They then used t-tests to calculate the differences in microbiota at the taxon level between the control and experimental groups.
The following bacterial taxa were less abundant in mice with simulated endometriosis than in mice without endometriosis.
Abundance in Group 1: decreased abundance in Mice who were injected with endometrial tissues
NCBI | Quality Control | Links |
---|---|---|
Bacteroidota | ||
Bacteroidia | ||
Bacteroidales |
Revision editor(s): Samara.Khan
Experiment 2
Subjects
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 4
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Source: Figure 4
Description: LDA coupled with effect size measurements identified the most differentially abundant taxa between the two groups
Abundance in Group 1: increased abundance in Mice who were injected with endometrial tissues
NCBI | Quality Control | Links |
---|---|---|
Bacillota |
Signature 2
Source: Figure 4
Description: LDA coupled with effect size measurements identified the most differentially abundant taxa between the two groups
Abundance in Group 1: decreased abundance in Mice who were injected with endometrial tissues
NCBI | Quality Control | Links |
---|---|---|
Alloprevotella | ||
Bacteroidia | ||
Bacteroidales | ||
Bacteroidota |