Molecular detection of intrauterine microbial colonization in women with endometriosis
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Khan KN, Fujishita A, Masumoto H, Muto H, Kitajima M, Masuzaki H, Kitawaki J
Journal
European journal of obstetrics, gynecology, and reproductive biology
Year
2016
Keywords:
16S rDNA metagenome assay, Cystic fluid, Endometriosis, Infection
OBJECTIVE: Increased intrauterine microbial colonization by bacteria culture method and occurrence of endometritis have been reported in women with endometriosis. Here we investigated microbial colonization in intrauterine environment and cystic fluid of women with and without endometriosis by molecular approach. STUDY DESIGN: This is a case-controlled biological study with a total of 32 women each with and without endometriosis. Among them, 16 each in these two groups of women received treatment with gonadotropin-releasing hormone agonist (GnRHa). Pattern of microbial colonization in endometrial swabs and endometrioma/non-endometrioma cystic fluid was examined using broad-range polymerase-chain reaction (PCR) amplification of bacteria targeting 16S rRNA gene (rDNA). After quantification of index PCR product, 16S rDNA metagenome sequence analysis was done by Illumina Miseq system. RESULTS: A wide proportion (0.01-97.8%) of multiple bacteria was detected in both endometrial swabs and cystic fluid collected from women with and without endometriosis. 16S metagenome assay indicated that proportion of Lactobacillacae was significantly decreased (p<0.01) and of Streptococcaceae, Staphylococaceae, Enterobacteriaceae was significantly increased (p<0.05 for each) in GnRHa-treated women with endometriosis than in GnRHa-untreated women. While bacteria culture method failed to detect a single colony, 16S metagenome assay could detect significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts. CONCLUSION: These findings indicate the occurrence of sub-clinical infection in intrauterine environment and in the cystic fluid of ovarian endometrioma. Additional side effect of GnRHa treatment in promoting silent intrauterine and/or ovarian infection should be considered.
Experiment 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Curated date: 2021/08/08
Curator: Samara.Khan
Revision editor(s): Samara.Khan, WikiWorks, Atrayees, Peace Sandy
Subjects
- Location of subjects
- Japan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Endometrium Tunica mucosa (endometrium),Tunica mucosa uteri,Uterine endometrium,Uterine mucosa,Uterine mucous membrane,Endometrium,endometrium
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Untreated control women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endometriosis patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients were diagnosed with endometriosis via laparoscopic surgery and stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM). 16 of the control patients and 16 of the endometriosis patients received GnRHa (gonadotropin releasing hormone agonist) treatment for 4-6 months.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 32
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Source: Figure 3
Description: Regardless of GnRHa treatment, patients with endometriosis had higher levels of streptococcaceae and moraxellaceae than those without endometriosis.
Abundance in Group 1: increased abundance in Endometriosis patients
| NCBI | Quality Control | Links |
|---|---|---|
| Streptococcaceae | ||
| Moraxellaceae |
Revision editor(s): Samara.Khan
Experiment 2
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Endometriosis patients not treated with GnRHa
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endometriosis patients treated with GnRHa
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients were diagnosed with endometriosis via laparoscopic surgery and stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM). 16 of the 32 endometriosis patients received GnRHa (gonadotropin releasing hormone agonist) treatment for 4-6 months.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Source: Figure 4
Description: Streptococcaceae, Staphylococaceae, Enterobacteriaceae were significantly increased (p<0.05 for each) in GnRHa-treated women with endometriosis than in GnRHa-untreated women.
Abundance in Group 1: increased abundance in Endometriosis patients treated with GnRHa
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Streptococcaceae | ||
| Staphylococcaceae |
Revision editor(s): Samara.Khan
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Source: Figure 4
Description: Lactobacillaceae was significantly decreased (p<0.01) in GnRHa-treated women with endometriosis compared to GnRHa-untreated women with endometriosis
Abundance in Group 1: decreased abundance in Endometriosis patients treated with GnRHa
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillaceae |
Revision editor(s): Samara.Khan
Experiment 3
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Differences from previous experiment shown
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Ovary Animal ovary,Female gonad,Female organism genitalia gonad,Female organism genitalia gonada,Female organism reproductive system gonad,Female organism reproductive system gonada,Female reproductive system gonad,Female reproductive system gonada,Genitalia of female organism gonad,Genitalia of female organism gonada,Gonad of female organism genitalia,Gonad of female organism reproductive system,Gonad of female reproductive system,Gonad of genitalia of female organism,Gonad of reproductive system of female organism,Gonada of female organism genitalia,Gonada of female organism reproductive system,Gonada of female reproductive system,Gonada of genitalia of female organism,Gonada of reproductive system of female organism,Ovaries,Ovarium,Ovum-producing ovary,Reproductive system of female organism gonad,Reproductive system of female organism gonada,Ovary,ovary
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Patients with non-endometrioma cysts
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Patients with ovarian endometrioma cysts
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with ovarian endometrioma had endometrial cysts in their ovaries. Researchers took samples of the fluid inside these cysts. Ovarian endometrioma is one of the many ways endometriosis manifests in the female reproductive system.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Source: Figure 5
Description: There was a significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts.
Abundance in Group 1: increased abundance in Patients with ovarian endometrioma cysts
| NCBI | Quality Control | Links |
|---|---|---|
| Streptococcaceae | ||
| Staphylococcaceae |
Revision editor(s): Samara.Khan
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-8-7
Source: Figure 5
Description: There was a significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts.
Abundance in Group 1: decreased abundance in Patients with ovarian endometrioma cysts
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillaceae |
Revision editor(s): Atrayees
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