Molecular detection of intrauterine microbial colonization in women with endometriosis
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Curated date: 2021/08/08
Curator: Samara.Khan
Revision editor(s): WikiWorks, Samara.Khan, Atrayees, Peace Sandy
Subjects
- Location of subjects
- Japan
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Endometrium Tunica mucosa (endometrium),Tunica mucosa uteri,Uterine endometrium,Uterine mucosa,Uterine mucous membrane,Endometrium,endometrium
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Untreated control women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endometriosis patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients were diagnosed with endometriosis via laparoscopic surgery and stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM). 16 of the control patients and 16 of the endometriosis patients received GnRHa (gonadotropin releasing hormone agonist) treatment for 4-6 months.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 32
- Group 1 sample size Number of subjects in the case (exposed) group
- 32
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Source: Figure 3
Description: Regardless of GnRHa treatment, patients with endometriosis had higher levels of streptococcaceae and moraxellaceae than those without endometriosis.
Abundance in Group 1: increased abundance in Endometriosis patients
NCBI | Quality Control | Links |
---|---|---|
Streptococcaceae | ||
Moraxellaceae |
Revision editor(s): Samara.Khan
Experiment 2
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Endometriosis patients not treated with GnRHa
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Endometriosis patients treated with GnRHa
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients were diagnosed with endometriosis via laparoscopic surgery and stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM). 16 of the 32 endometriosis patients received GnRHa (gonadotropin releasing hormone agonist) treatment for 4-6 months.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Signature 1
Source: Figure 4
Description: Streptococcaceae, Staphylococaceae, Enterobacteriaceae were significantly increased (p<0.05 for each) in GnRHa-treated women with endometriosis than in GnRHa-untreated women.
Abundance in Group 1: increased abundance in Endometriosis patients treated with GnRHa
NCBI | Quality Control | Links |
---|---|---|
Enterobacteriaceae | ||
Streptococcaceae | ||
Staphylococcaceae |
Revision editor(s): Samara.Khan
Signature 2
Source: Figure 4
Description: Lactobacillaceae was significantly decreased (p<0.01) in GnRHa-treated women with endometriosis compared to GnRHa-untreated women with endometriosis
Abundance in Group 1: decreased abundance in Endometriosis patients treated with GnRHa
NCBI | Quality Control | Links |
---|---|---|
Lactobacillaceae |
Revision editor(s): Samara.Khan
Experiment 3
Subjects
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Ovary Animal ovary,Female gonad,Female organism genitalia gonad,Female organism genitalia gonada,Female organism reproductive system gonad,Female organism reproductive system gonada,Female reproductive system gonad,Female reproductive system gonada,Genitalia of female organism gonad,Genitalia of female organism gonada,Gonad of female organism genitalia,Gonad of female organism reproductive system,Gonad of female reproductive system,Gonad of genitalia of female organism,Gonad of reproductive system of female organism,Gonada of female organism genitalia,Gonada of female organism reproductive system,Gonada of female reproductive system,Gonada of genitalia of female organism,Gonada of reproductive system of female organism,Ovaries,Ovarium,Ovum-producing ovary,Reproductive system of female organism gonad,Reproductive system of female organism gonada,Ovary,ovary
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Patients with non-endometrioma cysts
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Patients with ovarian endometrioma cysts
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients with ovarian endometrioma had endometrial cysts in their ovaries. Researchers took samples of the fluid inside these cysts. Ovarian endometrioma is one of the many ways endometriosis manifests in the female reproductive system.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 8
Lab analysis
Statistical Analysis
Signature 1
Source: Figure 5
Description: There was a significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts.
Abundance in Group 1: increased abundance in Patients with ovarian endometrioma cysts
NCBI | Quality Control | Links |
---|---|---|
Streptococcaceae | ||
Staphylococcaceae |
Revision editor(s): Samara.Khan
Signature 2
Source: Figure 5
Description: There was a significantly higher percentage of Streptococcaceae (p<0.01) and Staphylococaceae (p<0.05) in the cystic fluid derived from women with ovarian endometrioma comparing to that in cystic fluid collected from non-endometrioma cysts.
Abundance in Group 1: decreased abundance in Patients with ovarian endometrioma cysts
NCBI | Quality Control | Links |
---|---|---|
Lactobacillaceae |
Revision editor(s): Atrayees