Altered Composition of Microbiota in Women with Ovarian Endometrioma: Microbiome Analyses of Extracellular Vesicles in the Peritoneal Fluid

Study information

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33925708

DOI Digital object identifier for electronic documents.

10.3390/ijms22094608

URI

Authors

Lee SR, Lee JC, Kim SH, Oh YS, Chae HD, Seo H, Kang CS, Shin TS

Journal

International journal of molecular sciences

Year

2021

Keywords:

16S rDNA, endometriosis, extracellular vesicles, microbiome

Human microbiota refers to living microorganisms which colonize our body and crucially contribute to the metabolism of nutrients and various physiologic functions. According to recently accumulated evidence, human microbiota dysbiosis in the genital tract or pelvic cavity could be involved in the pathogenesis and/or pathophysiology of endometriosis. We aimed to investigate whether the composition of microbiome is altered in the peritoneal fluid in women with endometriosis. We recruited 45 women with histological evidence of ovarian endometrioma and 45 surgical controls without endometriosis. Following the isolation of extracellular vesicles from peritoneal fluid samples from women with and without endometriosis, bacterial genomic DNA was sequenced using next-generation sequencing of the 16S rDNA V3-V4 regions. Diversity analysis showed significant differences in the microbial community at phylum, class, order, family, and genus levels between the two groups. The abundance of Acinetobacter, Pseudomonas, Streptococcus, and Enhydrobacter significantly increased while the abundance of Propionibacterium, Actinomyces, and Rothia significantly decreased in the endometriosis group compared with those in the control group (p < 0.05). These findings strongly suggest that microbiome composition is altered in the peritoneal environment in women with endometriosis. Further studies are necessary to verify whether dysbiosis itself can cause establishment and/or progression of endometriosis.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1, WikiWorks

Subjects

Location of subjects: South Korea

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Uterovesical pouch Excavatio vesico-uterina,Excavatio vesicouterina,Excavatio vesiocuterina,Vesico-uterine pouch,Vesicouterine,Vesicouterine excavation,Vesicouterine pouch,Uterovesical pouch,uterovesical pouch

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Surgical controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Stage 3/4 endometriosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Stage 3/4 endometriosis patients that were diagnosed with endometriosis via laparoscopic surgery; stages were categorized according to the revised American Society for Reproductive Medicine scoring system (r-ASRM).

Group 0 sample size Number of subjects in the control (unexposed) group: 45

Group 1 sample size Number of subjects in the case (exposed) group: 45

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Patients were excluded if they had taken antibiotics 12 weeks prior to sample collection

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: PERMANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Richness Number of species: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Table 1 and Results section

Description: Differential microbial abundance between peritoneal fluid samples among women with endometriosis when compared to surgical controls.

Abundance in Group 1: decreased abundance in Stage 3/4 endometriosis patients

NCBI	Quality Control	Links
Actinomycetota
Actinomyces
Actinomycetaceae
Actinomycetales
Propionibacteriaceae
Propionibacterium
Rothia
Veillonellaceae

Revision editor(s): Samara.Khan, Claregrieve1

Signature 2

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Claregrieve1 on 2022/07/8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Table 1 and Results section

Description: Differential microbial abundance between peritoneal fluid samples among women with endometriosis when compared to surgical controls.

Abundance in Group 1: increased abundance in Stage 3/4 endometriosis patients

NCBI	Quality Control	Links
Acinetobacter
Enhydrobacter
Pseudomonadaceae
Pseudomonadales
Pseudomonas
Streptococcus

Revision editor(s): Samara.Khan, Claregrieve1