Microbiota composition and distribution along the female reproductive tract of women with endometriosis

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

32299442

DOI Digital object identifier for electronic documents.

10.1186/s12941-020-00356-0

URI

Authors

Wei W, Zhang X, Tang H, Zeng L, Wu R

Journal

Annals of clinical microbiology and antimicrobials

Year

2020

Keywords:

16S rRNA gene amplicon sequencing, Endometriosis, Microbial community composition, Microbial distribution

Endometriosis (EMS) is a multifactorial disease that affects 10%-15% women of reproductive age and is associated with chronic pelvic pain and infertility. The pathogenesis of EMS has not been consistently explained until now. In this study, we involved 36 endometriosis patients and 14 control subjects who performed laparoscopic surgery due to gynecological benign tumor. The samples from lower third of vagina (CL), posterior vaginal fornix (CU), cervical mucus (CV), endometrium (ET) and peritoneal fluid (PF), were collected and sequenced by 16S rRNA amplicon. The continuous change of the microbiota distribution was identified along the reproductive tract. The flora in lower reproductive tract (CL, CU) were dominated by Lactobacillus. Significant difference of the community diversity began showing in the CV of EMS patients and gradually increased upward the reproductive tract. It indicates the microbiota in cervical samples is expected to be an indicator for the risk of EMS. This study also highlights the decreasing of Lactobacillus in vaginal flora and the increasing of signature Operational Taxonomic Units (OTUs) in transaction zone (CV) and upper reproductive tract (ET, PF) of EMS patients, which reflect the alteration of microbial community associated with EMS, participation of specific colonized bacteria in the EMS pathogenesis and relationship between microbiota and development of disease.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8

Curated date: 2021/08/09

Curator: Samara.Khan

Revision editor(s): Samara.Khan, WikiWorks, Peace Sandy

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Lower part of vagina , Upper part of vagina , Cervical mucus Caudal vagina,Lower third of vagina,Lower vagina,Perineal part of vagina,Sinus vagina,Vagina lower part,Vaginal bulb,Lower part of vagina,lower part of vagina,Cranial vagina,Pelvic part of vagina,Upper third of vagina,Upper vagina,Vagina upper part,Upper part of vagina,upper part of vagina,Cervix mucus,Cervical mucus,cervical mucus

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Controls Subject undergoing laparoscopic surgery for benign tumors

Group 1 name Corresponds to the case (exposed) group for case-control studies: Endometriosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Women with endometriosis

Group 0 sample size Number of subjects in the control (unexposed) group: 14

Group 1 sample size Number of subjects in the case (exposed) group: 36

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 Months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Ion Torrent

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8

Curated date: 2024/02/08

Curator: Peace Sandy

Revision editor(s): Peace Sandy, WikiWorks

Source: Fig. 2

Description: The signature OTUs identified in the different sites of female reproductive tract were shown, including a vagina (CL), b posterior vaginal fornix (CU), c cervical mucus (CV), d endometrium (ET) and e peritoneal fluid (PF). Blue bar and purple bar note higher abundancy were occurred in EMS patients and healthy women, representatively. The signature OTUs were defined by Wilcoxon-rank sum test with p value < 0.05

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI	Quality Control	Links
Lactobacillus iners
Vagococcus
Arthrobacter
Pseudomonas viridiflava
Sphingobium
Comamonadaceae
Delftia
Dysgonomonas
Pseudomonadaceae
Erysipelotrichaceae
Caulobacteraceae
Paracoccus
Shewanella
Pseudomonas sp.
Micrococcaceae
Acinetobacter
Enterococcus
Leucobacter
Paenibacillus
Pseudomonas
Tissierellaceae
Hyphomicrobium

Revision editor(s): Peace Sandy, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-8

Curated date: 2024/02/08

Curator: Peace Sandy

Revision editor(s): Peace Sandy, WikiWorks

Source: Fig. 2

Description: The signature OTUs identified in the different sites of female reproductive tract were shown, including a vagina (CL), b posterior vaginal fornix (CU), c cervical mucus (CV), d endometrium (ET) and e peritoneal fluid (PF). Blue bar and purple bar note higher abundancy were occurred in EMS patients and healthy women, representatively. The signature OTUs were defined by Wilcoxon-rank sum test with p value < 0.05

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI	Quality Control	Links
Aerococcus
Lactobacillus
Prevotella
Lactobacillus sp.
Leptotrichiaceae
Mobiluncus
Coriobacteriaceae
Rhodanobacteraceae
Dialister
Mediterraneibacter gnavus
Megasphaera
Prevotella corporis
Parvimonas
Eubacteriales
Enterococcus casseliflavus
Hyphomicrobiales
Oscillospira
Macrococcoides caseolyticum
Pseudomonadaceae
Sphingobacterium multivorum
Arthrobacter
Enterobacteriaceae
Acinetobacter
Wolbachia
Agromyces
Sutterella

Revision editor(s): Peace Sandy, WikiWorks