Associations Between Endometriosis and Gut Microbiota

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Svensson A, Brunkwall L, Roth B, Orho-Melander M, Ohlsson B
Journal
Reproductive sciences (Thousand Oaks, Calif.)
Year
2021
Keywords:
Endometriosis, Gastrointestinal symptoms, Gut microbiota, Pathophysiology
The gut microbiota has been associated with many diseases, including endometriosis. However, very few studies have been conducted on this topic in human. This study aimed to investigate the association between endometriosis and gut microbiota. Women with endometriosis (N=66) were identified at the Department of Gynaecology and each patient was matched with three controls (N=198) from the general population. All participants answered questionnaires about socioeconomic data, medical history, and gastrointestinal symptoms and passed stool samples. Gut bacteria were analyzed using 16S ribosomal RNA sequencing, and in total, 58 bacteria were observed at genus level in both patients with endometriosis and controls. Comparisons of the microbiota between patients and controls and within the endometriosis cohort were performed. Both alpha and beta diversities were higher in controls than in patients. With the false discovery rate q<0.05, abundance of 12 bacteria belonging to the classes Bacilli, Bacteroidia, Clostridia, Coriobacteriia, and Gammaproteobacter differed significantly between patients and controls. Differences observed between patients with or without isolated ovarian endometriosis, involvement of the gastrointestinal tract, gastrointestinal symptoms, or hormonal treatment disappeared after calculation with false discovery rate. These findings indicate that the gut microbiota may be altered in endometriosis patients.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): WikiWorks, Claregrieve1, Samara.Khan, Peace Sandy

Subjects

Location of subjects
Sweden
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Healthy controls taken from a larger cohort study
Group 1 name Corresponds to the case (exposed) group for case-control studies
Endometriosis patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with a diagnosis of endometriosis, confirmed by laparoscopy or laparotomy.
Group 0 sample size Number of subjects in the control (unexposed) group
198
Group 1 sample size Number of subjects in the case (exposed) group
66

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V1-V3
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes
Matched on Factors on which subjects have been matched on in a case-control study
age, body mass index, sex, smoking status

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1, Folakunmi

Source: Table 2

Description: The following taxa were decreased among those with endometriosis compared to healthy controls.

Abundance in Group 1: decreased abundance in Endometriosis patients

NCBI Quality ControlLinks
Adlercreutzia
Bacteroidales
Barnesiellaceae
Coriobacteriaceae
Halanaerobiaceae
Lachnospira
Paraprevotella
Turicibacter

Revision editor(s): Samara.Khan, Claregrieve1, Folakunmi

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Table 2

Description: The following taxa were increased among those with endometriosis compared to healthy controls after a false-discovery rate adjustment

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI Quality ControlLinks
Oscillospira
Bacteroides
Parabacteroides
Enterobacteriaceae
Coprococcus
Lachnospiraceae

Revision editor(s): Samara.Khan, Claregrieve1

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): WikiWorks, Claregrieve1, Samara.Khan, Victoria

Differences from previous experiment shown

Subjects

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Supplementary Table 2

Description: Differential microbial abundance between endometriosis patients and healthy controls after a sensitivity analysis and adjustment for false-discovery rate.

Abundance in Group 1: decreased abundance in Endometriosis patients

NCBI Quality ControlLinks
Bacteroidales

Revision editor(s): Samara.Khan, Claregrieve1

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Supplementary Table 2

Description: Differential microbial abundance between endometriosis patients and healthy controls after a sensitivity analysis and adjustment for false-discovery rate.

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI Quality ControlLinks
Lachnospira
Oscillospira

Revision editor(s): Samara.Khan, Claregrieve1