Associations Between Endometriosis and Gut Microbiota

Study information

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

33660232

DOI Digital object identifier for electronic documents.

10.1007/s43032-021-00506-5

URI

Authors

Svensson A, Brunkwall L, Roth B, Orho-Melander M, Ohlsson B

Journal

Reproductive sciences (Thousand Oaks, Calif.)

Year

2021

Keywords:

Endometriosis, Gastrointestinal symptoms, Gut microbiota, Pathophysiology

The gut microbiota has been associated with many diseases, including endometriosis. However, very few studies have been conducted on this topic in human. This study aimed to investigate the association between endometriosis and gut microbiota. Women with endometriosis (N=66) were identified at the Department of Gynaecology and each patient was matched with three controls (N=198) from the general population. All participants answered questionnaires about socioeconomic data, medical history, and gastrointestinal symptoms and passed stool samples. Gut bacteria were analyzed using 16S ribosomal RNA sequencing, and in total, 58 bacteria were observed at genus level in both patients with endometriosis and controls. Comparisons of the microbiota between patients and controls and within the endometriosis cohort were performed. Both alpha and beta diversities were higher in controls than in patients. With the false discovery rate q<0.05, abundance of 12 bacteria belonging to the classes Bacilli, Bacteroidia, Clostridia, Coriobacteriia, and Gammaproteobacter differed significantly between patients and controls. Differences observed between patients with or without isolated ovarian endometriosis, involvement of the gastrointestinal tract, gastrointestinal symptoms, or hormonal treatment disappeared after calculation with false discovery rate. These findings indicate that the gut microbiota may be altered in endometriosis patients.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1, WikiWorks, Peace Sandy

Subjects

Location of subjects

Sweden

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Endometriosis endometriosis,Endometriosis (clinical),endometriosis (disease),Endometriosis (disorder),Endometriosis (morphologic abnormality),ENDOMETRIOSIS NEC,Endometriosis NOS,Endometriosis NOS (disorder),Endometriosis of other specified sites,Endometriosis, site unspecified,Endometriosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Healthy controls taken from a larger cohort study

Group 1 name Corresponds to the case (exposed) group for case-control studies

Endometriosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Patients with a diagnosis of endometriosis, confirmed by laparoscopy or laparotomy.

Group 0 sample size Number of subjects in the control (unexposed) group

198

Group 1 sample size Number of subjects in the case (exposed) group

66

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V1-V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Matched on Factors on which subjects have been matched on in a case-control study

age, body mass index, sex, smoking status

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

decreased

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1, Folakunmi

Source: Table 2

Description: The following taxa were decreased among those with endometriosis compared to healthy controls.

Abundance in Group 1: decreased abundance in Endometriosis patients

NCBI	Quality Control	Links
Adlercreutzia
Bacteroidales
Barnesiellaceae
Coriobacteriaceae
Halanaerobiaceae
Lachnospira
Paraprevotella
Turicibacter

Revision editor(s): Samara.Khan, Claregrieve1, Folakunmi

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Table 2

Description: The following taxa were increased among those with endometriosis compared to healthy controls after a false-discovery rate adjustment

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI	Quality Control	Links
Oscillospira
Bacteroides
Parabacteroides
Enterobacteriaceae
Coprococcus
Lachnospiraceae

Revision editor(s): Samara.Khan, Claregrieve1

Experiment 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1, WikiWorks

Differences from previous experiment shown

Subjects

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

6 months

Lab analysis

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

Not specified

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness

decreased

Signature 1

EditHistoryDelete

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Supplementary Table 2

Description: Differential microbial abundance between endometriosis patients and healthy controls after a sensitivity analysis and adjustment for false-discovery rate.

Abundance in Group 1: decreased abundance in Endometriosis patients

NCBI	Quality Control	Links
Bacteroidales

Revision editor(s): Samara.Khan, Claregrieve1

Signature 2

EditHistoryDelete

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Your review change was submitted

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/08/11

Curator: Samara.Khan

Revision editor(s): Samara.Khan, Claregrieve1

Source: Supplementary Table 2

Description: Differential microbial abundance between endometriosis patients and healthy controls after a sensitivity analysis and adjustment for false-discovery rate.

Abundance in Group 1: increased abundance in Endometriosis patients

NCBI	Quality Control	Links
Lachnospira
Oscillospira

Revision editor(s): Samara.Khan, Claregrieve1