Changes in gut microbiota and plasma inflammatory factors across the stages of colorectal tumorigenesis: a case-control study

Study information

Reviewed Marked as Reviewed by Fatima on 2022/10/6

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30157754

DOI Digital object identifier for electronic documents.

10.1186/s12866-018-1232-6

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114884/

Authors

Zhang Y, Yu X, Yu E, Wang N, Cai Q, Shuai Q, Yan F, Jiang L, Wang H, Liu J, Chen Y, Li Z, Jiang Q

Journal

BMC microbiology

Year

2018

Keywords:

Colorectal cancer (CRC), Correlation analysis, Gut microbiota, Plasma inflammatory factors

BACKGROUND: Colorectal cancer (CRC) is a common malignant gastrointestinal tumor. In China, CRC is the 5th most commonly diagnosed cancer. The vast majority of CRC cases are sporadic and evolve with the adenoma-carcinoma sequence. There is mounting evidence indicating that gut microbiota and inflammation play important roles in the development of CRC although study results are not entirely consistent. In the current study, we investigated the changes in the CRC-associated bacteria and plasma inflammatory factors and their relationships based on data from a case-control study of Han Chinese. We included 130 initially diagnosed CRC patients, 88 advanced colorectal adenoma patients (A-CRA), 62 patients with benign intestinal polyps and 130 controls. RESULTS: Fecal microbiota composition was obtained using 16S ribosomal DNA (16S rDNA) sequencing. PCOA analysis showed structural differences in microbiota among the four study groups (P = 0.001, Unweighted Unifrac). Twenty-four CRC-associated bacteria were selected by a two-step statistical method and significant correlations were observed within these microbes. CRC-associated bacteria were found to change with the degree of malignancy. Plasma C-reactive protein (CRP) and soluble tumor necrosis factor II (sTNFR-II) displayed significant differences among the four study groups and increased with adenoma-carcinoma sequence. The correlations of CRP and sTNFR-II with several CRC-associated microbes were also explored. CONCLUSIONS: CRC-associated species and plasma inflammatory factors tended to change along the adenoma-carcinoma sequence. Several CRC-associated bacteria were correlated with CRP and sTNFR-II. It is likely that gut microbiome and inflammation gradually form a microenvironment that is associated with CRC development.

Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2022/01/19

Curator: Itslanapark

Revision editor(s): Itslanapark, Claregrieve1, WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: CRC patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients diagnosed with colorectal cancer

Group 0 sample size Number of subjects in the control (unexposed) group: 130

Group 1 sample size Number of subjects in the case (exposed) group: 130

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 6 months

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: Zero-Inflated Beta Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: age, alcohol drinking, body mass index, sex, smoking status

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2022/01/20

Curator: Itslanapark

Revision editor(s): Itslanapark, Claregrieve1, Atrayees

Source: Table S1

Description: CRC-associated microbiota in CRC patients compared with controls

Abundance in Group 1: increased abundance in CRC patients

NCBI	Quality Control	Links
Campylobacter rectus
Dialister pneumosintes
Eggerthella lenta
Fusobacterium nucleatum
Gemella morbillorum
Parvimonas micra
Peptostreptococcus stomatis
Porphyromonas asaccharolytica
Scriptaphyosemion liberiense
Solobacterium moorei
[Clostridium] scindens
[Clostridium] symbiosum
[Ruminococcus] torques

Revision editor(s): Itslanapark, Claregrieve1, Atrayees

Signature 2

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/10/6

Curated date: 2022/01/20

Curator: Itslanapark

Revision editor(s): Itslanapark, Claregrieve1, Aiyshaaaa

Source: Table S1

Description: CRC-associated microbiota in CRC patients compared with controls

Abundance in Group 1: decreased abundance in CRC patients

NCBI	Quality Control	Links
Agathobaculum desmolans
Anaerobutyricum hallii
Anaerostipes hadrus
Blautia faecis
Coprococcus comes
Fusicatenibacter saccharivorans
Lachnospira eligens
Roseburia faecis
Streptococcus salivarius
[Ruminococcus] lactaris

Revision editor(s): Itslanapark, Claregrieve1, Aiyshaaaa