Gut microbiota composition is correlated to grid floor induced stress and behavior in the BALB/c mouse/Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects

Denmark

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Caecum Blind intestine,Blindgut,Caeca,Caecum,Ceca,Cecum,Intestinum caecum,Intestinum crassum caecum,Intestinum crassum cecum,caecum

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Stimulus or stress design stimulus_or_stress_design,Stimulus or stress design,stimulus or stress design

Group 0 name Corresponds to the control (unexposed) group for case-control studies

control

Group 1 name Corresponds to the case (exposed) group for case-control studies

grid floor induced stress

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

cases were exposed to a stress factor which is a grid floor exposure for two weeks. this implies that the use of grid floor is a sufficient stressor for inducing a depression-like behavioral state in the TST.

Group 0 sample size Number of subjects in the control (unexposed) group

14

Group 1 sample size Number of subjects in the case (exposed) group

14

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Signature 1

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Your review change was submitted

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Fatima Zohra

Revision editor(s): WikiWorks

Source: Table 2

Description: Significantly different taxa between control mice and grid floor housed mice

Abundance in Group 1: increased abundance in grid floor induced stress

NCBI	Quality Control	Links
Odoribacter
Alistipes

Revision editor(s): WikiWorks