Diversified microbiota of meconium is affected by maternal diabetes status/Experiment 2

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects

United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Meconium Meconium,meconium

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Gestational diabetes diabetes in pregnancy,GDM,gestational diabetes,gestational diabetes mellitus,maternal gestational diabetes mellitus,Gestational diabetes

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Healthy baby meconium

Group 1 name Corresponds to the case (exposed) group for case-control studies

Baby meconium with GDM mothers

Group 0 sample size Number of subjects in the control (unexposed) group

13

Group 1 sample size Number of subjects in the case (exposed) group

5

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

9 months

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

PacBio RS

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

No

Signature 1

EditHistoryDelete

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Your review change was submitted

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Rimsha Azhar

Revision editor(s): WikiWorks

Source: Text + Figure 4

Description: Significant difference between neonates from mothers with different diabetes states

Abundance in Group 1: decreased abundance in Baby meconium with GDM mothers

NCBI	Quality Control	Links
Betaproteobacteria
Brucellaceae
Alcaligenaceae
Comamonadaceae
Xanthobacteraceae
Alcaligenes
Cupriavidus
Comamonas
Delftia
Stenotrophomonas

Revision editor(s): WikiWorks