Sex effects in the association between airway microbiome and asthma

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Chen R, Wang L, Koch T, Curtis V, Yin-DeClue H, Handley SA, Shan L, Holtzman MJ, Castro M, Wang L
Journal
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
Year
2020
BACKGROUND: Sex differences exist in asthma susceptibility and severity. Accumulating evidence has linked airway microbiome dysbiosis to asthma, and airway microbial communities have been found to differ by sex. However, whether sex modifies the link between airway microbiome and asthma has not been investigated. OBJECTIVE: To evaluate sex effects in the association between airway microbiome and asthma. METHODS: We analyzed induced sputum samples from 47 subjects (n = 23 patients with asthma and n = 24 normal controls) using 16S ribosomal RNA gene sequencing methods. The bacterial composition was analyzed for sex differences. Bacterial associations with asthma were assessed for each sex at the core taxa and genus levels. RESULTS: The microbiome in induced sputum differed in women vs men at the community level. A total of 5 core bacterial taxa were found in all samples. No sex-specific core taxa were detected. The most abundant core taxon, Streptococcus salivarius, was significantly enriched in women than in men (P = .02). Within each sex, individuals with relatively lower abundance of S salivarius were more likely to have asthma (P = .006). For both sexes, increased Lactobacillus species were found in sputum samples of patients with patients compared with normal controls (adjusted P = .01). Haemophilus species were associated with asthma in men and not in women. CONCLUSION: The airway microbiome differed by sex, and sex effects exist in the association of airway microbial markers and asthma. Future airway microbiome studies may yield better resolution if the context of specific sex is considered. The airway microbiome is a potential mechanism driving sex differences in asthma.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/12/11

Curator: Joyessa

Revision editor(s): Joyessa, Claregrieve1, WikiWorks, Peace Sandy

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Sputum Expectoration,Sputum,sputum
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Asthma Airway hyperreactivity,asthma,Asthma (disorder),Asthma NOS,Asthma NOS (disorder),ASTHMA NOS W (AC) EXAC,Asthma unspecified,Asthma unspecified (disorder),Asthma, Bronchial,Asthma, unspecified,Asthma, unspecified type, with acute exacerbation,Asthma, unspecified type, without mention of status asthmaticus,Asthmas,Asthmatic,BHR - Bronchial hyperreactivity,Bronchial asthma,Bronchial Hyperreactivities,Bronchial hyperreactivity,bronchial hyperreactivity,Bronchial hyperresponsiveness,Bronchial hypersensitivity,chronic obstructive asthma,chronic obstructive asthma with acute exacerbation,chronic obstructive asthma with status asthmaticus,DUST PNEUMONOPATHY NEC,Exercise induced asthma,exercise induced asthma,Exercise-induced asthma,exercise-induced asthma,Exercise-induced asthma (disorder),Hyperreactive airway disease,Hyperreactive airways disease,Hyperreactivities, Bronchial,Hyperreactivity, Bronchial,Other forms of asthma,Pneumonopathy due to inhalation of other dust,Pneumopathy due to inhalation of other dust,Pneumopathy due to inhalation of other dust (disorder),Pneumopathy due to inhalation of other dust NOS,Pneumopathy due to inhalation of other dust NOS (disorder),Asthma
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Female
Group 1 name Corresponds to the case (exposed) group for case-control studies
Male
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Male participants
Group 0 sample size Number of subjects in the control (unexposed) group
32
Group 1 sample size Number of subjects in the case (exposed) group
15
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
1 month

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V1-V2
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/12/12

Curator: Joyessa

Revision editor(s): Joyessa, Claregrieve1

Source: Figure 1, Figure 2

Description: Differential microbial abundance between men and women

Abundance in Group 1: decreased abundance in Male

NCBI Quality ControlLinks
Actinomycetota
Bacillota
Streptococcus salivarius

Revision editor(s): Joyessa, Claregrieve1

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2021/12/14

Curator: Joyessa

Revision editor(s): Joyessa, Claregrieve1

Source: Figure 1

Description: Differential microbial abundance between men and women

Abundance in Group 1: increased abundance in Male

NCBI Quality ControlLinks
Bacteroidota

Revision editor(s): Joyessa, Claregrieve1

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2022/08/31

Curator: Claregrieve1

Revision editor(s): Claregrieve1, WikiWorks, Victoria

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Female normal controls
Group 1 name Corresponds to the case (exposed) group for case-control studies
Female participants with asthma
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Females participants with asthma
Group 0 sample size Number of subjects in the control (unexposed) group
17

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/10/20

Curated date: 2022/08/31

Curator: Claregrieve1

Revision editor(s): Claregrieve1

Source: Figure 2b

Description: Differential microbial abundance between female participants with asthma and female normal controls

Abundance in Group 1: decreased abundance in Female participants with asthma

NCBI Quality ControlLinks
Streptococcus salivarius

Revision editor(s): Claregrieve1

Signature 3

Reviewed Marked as Reviewed by Claregrieve1 on 2022/08/31

Curated date: 2022/08/31

Curator: Claregrieve1

Revision editor(s): Claregrieve1

Source: Figure 3b

Description: Differential microbial abundance between female participants with asthma and female normal controls

Abundance in Group 1: increased abundance in Female participants with asthma

NCBI Quality ControlLinks
Lactobacillus

Revision editor(s): Claregrieve1