Exposure to Titanium Dioxide Nanoparticles During Pregnancy Changed Maternal Gut Microbiota and Increased Blood Glucose of Rat

Study information

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

30656437

DOI Digital object identifier for electronic documents.

10.1186/s11671-018-2834-5

URI

Authors

Mao Z, Li Y, Dong T, Zhang L, Zhang Y, Li S, Hu H, Sun C, Xia Y

Journal

Nanoscale research letters

Year

2019

Keywords:

Gut microbiota, Increased fasting blood glucose, Pregnancy exposure, TiO2 NPs

Titanium dioxide nanoparticles (TiO2 NPs) were used worldwide for decades, and pregnant women are unable to avoid exposing to them. Studies revealed that TiO2 NPs could kill many kinds of bacteria, but whether they would affect the composition of gut microbiota, especially during pregnancy, was seldom reported. And, what adverse effects may be brought to pregnant females was also unknown. In this study, we established the prenatal exposure model of rats to explore the effects of TiO2 NPs on gut microbiota. We observed an increasing trend, but not a significant change of alpha-diversity among control and exposure groups at gestation day (GD) 10 and GD 17 during normal pregnancy process. Each different time point had unique gut microbiota operational taxonomic units (OTUs) characteristics. The abundance of Ellin6075 decreased at GD 10 and GD 17, Clostridiales increased at GD 10, and Dehalobacteriaceae decreased at GD 17 after TiO2 NPs exposure. Further phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) prediction indicated that the type 2 diabetes mellitus related genes were enhanced, and taurine metabolism was weakened at the second-trimester. Further study showed that the rats' fasting blood glucose levels significantly increased at GD 10 (P < 0.05) and GD 17 (P < 0.01) after exposure. Our study pointed out that TiO2 NPs induced the alteration of gut microbiota during pregnancy and increased the fasting blood glucose of pregnant rats, which might increase the potential risk of gestational diabetes of pregnant women.

Experiment 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: WikiWorks

Revision editor(s): WikiWorks, Victoria

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Rattus norvegicus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Titanium dioxide nanoparticle titanium dioxide nanoparticles,Titanium dioxide nanoparticle,titanium dioxide nanoparticle

Group 0 name Corresponds to the control (unexposed) group for case-control studies: control pregnant rats at gestational day 10

Group 1 name Corresponds to the case (exposed) group for case-control studies: pregnant rats exposed to titanium dioxide nanoparticles

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: The female rats were daily gavage administrated with 5 mg/kg bw/day of TiO2 NPs from the 5th to 18th day after pregnancy.

Group 0 sample size Number of subjects in the control (unexposed) group: 4

Group 1 sample size Number of subjects in the case (exposed) group: 4

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09

Curated date: 2021/01/10

Curator: Yaseen Javaid

Revision editor(s): WikiWorks

Source: Figure 4

Description: Exposure to Titanium Dioxide Nanoparticles During Pregnancy Changed Maternal Gut Microbiota of preganat rats

Abundance in Group 1: increased abundance in pregnant rats exposed to titanium dioxide nanoparticles

NCBI	Quality Control	Links
Eubacteriales

Revision editor(s): WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Fatima on 2021/07/28

Curated date: 2021/01/10

Curator: Yaseen Javaid

Revision editor(s): Fatima, WikiWorks

Source: Figure 4

Description: Exposure to Titanium Dioxide Nanoparticles During Pregnancy Changed Maternal Gut Microbiota of preganat rats

Abundance in Group 1: decreased abundance in pregnant rats exposed to titanium dioxide nanoparticles

NCBI	Quality Control	Links
bacterium Ellin6075

Revision editor(s): Fatima, WikiWorks