Twice-daily application of HIV microbicides alter the vaginal microbiota
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Vagina Distal oviductal region,Distal portion of oviduct,Vaginae,Vagina
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Human immunodeficiency virus AIDS virus,HIV,Human immunodeficiency virus
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy females who recieved universal placebo
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Healthy females who recieved two vaginal HIV microbicidal gels
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy females were treated with two gels twice daily over 13.5 days.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 10
- Group 1 sample size Number of subjects in the case (exposed) group
- 25
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V5-V6
Signature 1
Source: Figure 1
Description: Complete interconnection Five primary bacterial community state categories were discovered using hierarchical clustering approaches (CSTs). Different Lactobacillus species dominated three CSTs: L. crispatus (CST I), L. iners (CST III), and L. gasseri (CST IV) (CST II). Higher proportions and types of anaerobic bacteria such as Atopobium, Prevotella, Megaspherae, Sneathia, and Mobiluncus, as well as Gardnerella (the latter in proportions ranging from 0.2 percent to 9.9 percent) were found in vaginal bacterial communities from samples that clustered in CST IV-B, whereas CST IV-A Schematicoftheclinicalstudydesign. Green recommends using the gel items twice a day. Idealizedmensesareindicatedinred. At visits 2, 3, 4, 5, and 6, vaginal swabs were taken.
Abundance in Group 1: increased abundance in Healthy females who recieved two vaginal HIV microbicidal gels
NCBI | Quality Control | Links |
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Atopobium | ||
Enterococcus | ||
Escherichia | ||
Gardnerella | ||
Mobiluncus | ||
Prevotella | ||
Sneathia | ||
Streptococcus | ||
Megasphaera |