Effect of mother's weight on infant's microbiota acquisition, composition, and activity during early infancy: a prospective follow-up study initiated in early pregnancy
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Collado MC, Isolauri E, Laitinen K, Salminen S
Journal
The American journal of clinical nutrition
Year
2010
BACKGROUND: It has been reported that deviations in gut microbiota composition may predispose toward obesity, and specific groups of commensal gut bacteria may harvest energy from food more efficiently than others. Alterations in microbiota compositions of mothers may be transferred to infants and lead to an increased risk of overweight. OBJECTIVE: We analyzed the fecal microbiota composition of infants of overweight and normal-weight mothers and assessed the relations of weight and excessive weight gain of mothers during pregnancy on the microbiota of infants. DESIGN: Mothers (n = 16) whose prepregnancy body mass index (BMI; in kg/m²) was ≥25 were selected with their infants from a prospective follow-up study of 256 women. Women with a BMI <25 (n = 26) and their infants served as control subjects. At the ages of 1 and 6 mo, infant stool samples were available for the analysis of microbiota composition by fluorescence in situ hybridization combined with flow cytometry and quantitative real-time polymerase chain reaction. RESULTS: Infants' fecal microbial composition was related to the weight and weight gain of their mothers during pregnancy. Fecal Bacteroides and Staphylococcus concentrations were significantly higher in infants of overweight mothers during the first 6 mo. Higher weights and BMIs of mothers were related to higher concentrations of Bacteroides, Clostridium, and Staphylococcus and lower concentrations of the Bifidobacterium group. Prevalences of Akkermansia muciniphila, Staphylococcus, and Clostridium difficile groups were lower in infants of normal-weight mothers and of mothers with normal weight gains during pregnancy. CONCLUSION: The composition and development of infant gut microbiota are influenced by BMI, weight, and weight gain of mothers during pregnancy.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- Finland
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- infants of normal weight mother at age 1 month
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- infants of overweight mothers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- mothers whose prepregnancy body mass index (BMI; in kg/m2) was >25
- Group 0 sample size Number of subjects in the control (unexposed) group
- 26
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- RT-qPCR
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2 & Tale 4
Description: Mirobial groups in feacal samples at 1 and 6 months of age according to maternal weight gain during pregnancy and BMI
Abundance in Group 1: decreased abundance in infants of overweight mothers
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Prevotella |
Revision editor(s): WikiWorks
Experiment 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- infants of normal weight mother at age 6 month
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2 & Tale 4
Description: Mirobial groups in feacal samples at 1 and 6 months of age according to maternal weight gain during pregnancy and BMI
Abundance in Group 1: increased abundance in infants of overweight mothers
| NCBI | Quality Control | Links |
|---|---|---|
| Hathewaya histolytica | ||
| Bifidobacterium catenulatum |
Revision editor(s): WikiWorks
Experiment 3
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- infants of normal maternal weight gain mothers at age 1 month
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- infants of excessive maternal weight gain mothers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Weight gain >16.0 kg in women with BMI (in kg/m2) <25 and >11.5 kg in women with BMI >25 was considered excessive
- Group 0 sample size Number of subjects in the control (unexposed) group
- 20
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Bacterial counts in infant fecal samples at 1 and 6 months of age according to maternal weight gain during pregnancy
Abundance in Group 1: decreased abundance in infants of excessive maternal weight gain mothers
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Prevotella |
Revision editor(s): WikiWorks
Experiment 4
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- infants of normal maternal weight gain mothers at age 6 months
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Bacterial counts in infant fecal samples at 1 and 6 months of age according to maternal weight gain during pregnancy
Abundance in Group 1: increased abundance in infants of excessive maternal weight gain mothers
| NCBI | Quality Control | Links |
|---|---|---|
| Hathewaya histolytica |
Revision editor(s): WikiWorks
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