Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Lwaldron on 2022/07/1
Curated date: 2022/01/28
Curator: Kwekuamoo
Revision editor(s): LGeistlinger, Lwaldron, Rimsha, Kwekuamoo, Peace Sandy
Subjects
- Location of subjects
- France
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Irritable bowel syndrome [X]Psychogenic IBS,Adaptive colitis,Colitides, Mucous,Colitis, Mucous,Colon spasm,Colon, Irritable,Functional bowel disease,IBD,IBS,IBS - Irritable bowel syndrome,IC - Irritable colon,Irritable bowel,Irritable bowel - IBS,irritable bowel syndrome,Irritable Bowel Syndromes,Irritable Colon,irritable colon,Irritable colon (disorder),Irritable colon - Irritable bowel syndrome,Irritable colon syndrome,Membranous colitis,Mucous Colitides,Mucous colitis,mucus colitis,Nervous colitis,Psychogenic IBS,psychogenic IBS,Spastic colitis,Spastic colon,spastic colon,Syndrome, Irritable Bowel,Syndromes, Irritable Bowel,Irritable bowel syndrome
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Healthy Control
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- constipated-IBS
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The 14 women IBS patients (age range: 36–59, mean age: 48) included in this study fulfilled the Rome II criteria for IBS. They were classified as IBS with constipation (C-IBS) by a questionnaire following the Rome II subgrouping criteria which have been recognized as valid also in the Rome III criteria.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 12
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Exclusion criteria included organic intestinal disease, other systemic disease, previous abdominal surgery, lactation, pregnancy, dementia or inadequate cooperative capability and antibiotic therapy during the previous 2 months.
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- T-Test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- Matched on Factors on which subjects have been matched on in a case-control study
- sex
Signature 1
Reviewed Marked as Reviewed by Rimsha on 2022/04/6
Source: Figure 1a, 1b
Description: FISH analysis of main bacterial taxonomic groups in faeces of healthy and IBS patients
Abundance in Group 1: decreased abundance in constipated-IBS
| NCBI | Quality Control | Links |
|---|---|---|
| Bifidobacterium | ||
| Eubacterium | ||
| Roseburia |
Revision editor(s): Rimsha
Signature 2
Reviewed Marked as Reviewed by Rimsha on 2022/04/6
Source: Table 1
Description: Comparison of the population levels of microbial communities involved in carbohydrate metabolism in faecel samples from healthy and C-IBS individials
Abundance in Group 1: increased abundance in constipated-IBS
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae |
Revision editor(s): Rimsha
Signature 3
Reviewed Marked as Reviewed by Rimsha on 2022/04/6
Source: Table 1
Description: Comparison of the population levels of microbial communities involved in carbohydrate metabolism in faecel samples from healthy and C-IBS individials
Abundance in Group 1: decreased abundance in constipated-IBS
| NCBI | Quality Control | Links |
|---|---|---|
| Lactobacillus | ||
| Bifidobacterium |
