Comparison of Microbiota in Patients Treated by Surgery or Chemotherapy by 16S rRNA Sequencing Reveals Potential Biomarkers for Colorectal Cancer Therapy

Study information

Reviewed Marked as Reviewed by Rimsha on 2022/05/10

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

30065719

DOI Digital object identifier for electronic documents.

10.3389/fmicb.2018.01607

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057110/

Authors

Deng X, Li Z, Li G, Li B, Jin X, Lyu G

Journal

Frontiers in microbiology

Year

2018

Keywords:

16S rRNA sequencing, bacterial diversity, chemotherapy, colorectal cancer, surgery

Colorectal cancer (CRC) is the third most diagnosed cancer worldwide due to its high difficulty in early diagnosis, high mortality rate and short life span. Recent publications have demonstrated the involvement of the commensal gut microbiota in the initiation, progression and chemoresistance of CRC. However, this microbial community has not been explored within CRC patients after anti-cancer treatments. To this end, we performed next generation sequencing-based metagenomic analysis to determine the composition of the microbiota in CRC patients after anti-cancer treatments. The microbial 16S rRNA genes were analyzed from a total of 69 fecal samples from four clinical groups, including healthy individuals, CRC patients, and CRC patients treated with surgery or chemotherapy. The findings suggested that surgery greatly reduced the bacterial diversity of the microbiota in CRC patients. Moreover, Fusobacterium nucleatum were shown to confer chemoresistance during CRC therapy, and certain bacterial strains or genera, such as the genus Sutterella and species Veillonella dispar, were specifically associated with CRC patients who were treated with chemotherapeutic cocktails, suggesting their potential relationships with chemoresistance. These candidate bacterial genera or strains may have the ability to enhance the dosage response to conventional chemotherapeutic cocktails or reduce the side effects of these cocktails. A combination of common CRC risk factors, such as age, gender and BMI, identified in this study improved our understanding of the microbial community and its compositional variation during anti-cancer treatments. However, the underlying mechanisms of these microbial candidates remain to be investigated in animal models. Taken together, the findings of this study indicate that fecal microbiome-based approaches may provide additional methods for monitoring and optimizing anti-cancer treatments.

Experiment 1

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Reviewed Marked as Reviewed by Rimsha on 2022/05/10

Curated date: 2022/02/04

Curator: Itslanapark

Revision editor(s): Rimsha, Itslanapark, WikiWorks

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy individuals
Group 1 name Corresponds to the case (exposed) group for case-control studies: CRC patients
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: patients diagnosed with colorectal cancer
Group 0 sample size Number of subjects in the control (unexposed) group: 33
Group 1 sample size Number of subjects in the case (exposed) group: 31

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V4-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Statistical test: PERMANOVA

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.001
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Signature 1

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Reviewed Marked as Reviewed by Rimsha on 2022/05/10

Curated date: 2022/02/11

Curator: Itslanapark

Revision editor(s): Rimsha, Itslanapark

Source: Figure 6

Description: crc patients after chemotherapy

Abundance in Group 1: decreased abundance in CRC patients

NCBI	Quality Control	Links
Actinobacillus
Alcaligenaceae
Anaerostipes
Bacilli
Bacteroides ovatus
Bacteroides uniformis
Bacteroidota
Bacteroidia
Blautia producta
Citrobacter
Clostridia
Clostridiaceae
Clostridiales bacterium
Clostridium
Coprococcus
Cyanobacteriota
Dorea
Erwinia
Erysipelotrichaceae
Erysipelotrichales
Erysipelotrichia
Faecalibacterium
Faecalibacterium prausnitzii
Bacillota
Haemophilus
Lachnospira
Lachnospiraceae
Lactobacillales
Leptotrichia
Leptotrichiaceae
Neisseria
Oribacterium
Pasteurellales
Phascolarctobacterium
Phocaeicola plebeius
Prevotella
Prevotellaceae
Roseburia
Roseburia faecis
Ruminococcus flavefaciens
Selenomonas
Streptococcaceae
Streptococcus
Streptophyta
Sutterella
Veillonella
Veillonella dispar
Veillonella parvula
Veillonellaceae
[Ruminococcus] gnavus
uncultured Bacteroidetes bacterium

Revision editor(s): Rimsha, Itslanapark