Dysbiosis, gut barrier dysfunction and inflammation in dementia: a pilot study/Experiment 4

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Reviewed Marked as Reviewed by Lwaldron on 2022/03/28

Curated date: 2022/03/28

Curator: Lwaldron

Revision editor(s): Lwaldron, LGeistlinger, WikiWorks

Subjects

Location of subjects
Austria
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Dementia dementia,dementia (disease),Dementia
Group 0 name Corresponds to the control (unexposed) group for case-control studies
control, moderate, and severe dementia
Group 1 name Corresponds to the case (exposed) group for case-control studies
mild dementia
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
MMSE 20-26: The Mini-Mental State Examination [19] and the clock drawing test [20] were used to quantify cognitive function. We classified cognitive dysfunction according to the German S3-guideline on Dementia 2016 as MMSE 0–9: severe; MMSE 10–19: moderate; MMSE 20–26: mild; MMSE 27–30: no dementia [21].
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
4 weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V1-V2
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
Matched on Factors on which subjects have been matched on in a case-control study
age, sex

Alpha Diversity

Chao1 Abundance-based estimator of species richness
unchanged
Simpson Estimator of species richness and species evenness: more weight on species evenness
unchanged

Signature 1

Reviewed Marked as Reviewed by Lwaldron on 2022/03/28

Curated date: 2022/03/28

Curator: Lwaldron

Revision editor(s): Lwaldron

Source: Figure 4A (mild)

Description: These are "biomarkers" of mild dementia that are lower abundance in controls, moderate, and severe dementia.

Abundance in Group 1: increased abundance in mild dementia

NCBI Quality ControlLinks
Faecalibacterium prausnitzii
Faecalibacterium
Fusicatenibacter
Lachnospira eligens

Revision editor(s): Lwaldron