Effect of electronic cigarette and tobacco smoking on the human saliva microbial community
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Wang X, Mi Q, Yang J, Guan Y, Zeng W, Xiang H, Liu X, Yang W, Yang G, Li X, Cui Y, Gao Q
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Year
2022
Keywords:
Community profiles, E-cigarettes, Oral microbial flora, Smoking
Increasing evidence demonstrated the oral microbial community profile characteristics affected by conventional cigarettes smoking, but few studies focus on oral microbiome in response to electronic cigarettes (E-cigarettes). This study aimed to investigate the effect of E-cigarettes on the oral microbiome and to describe the difference of oral community profiles between E-cigarette smokers and tobacco smokers. 16S rRNA V4 gene sequencing was performed to investigate the oral microbial profiles of 5 E-cigarette smokers, 14 tobacco smokers, 8 quitting tobacco smokers, and 6 nonsmokers. The Chao1, ACE, and Shannon diversity indexes increased significantly in saliva samples collected from E-cigarette smokers and tobacco smokers compared to the non-smokers, and no significant difference was found in alpha diversity between E-cigarette smokers and tobacco smokers. The main phyla Proteobacteria, Firmicutes, Bacteroidetes, and Fusobacteria and major genera Neisseria, Streptococcus, Prevotellaceae, Fusobacterium, and Porphyromonas dominated in the smoking groups, while Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Fusobacteria became the dominant phyla along with the genera Corynebacterium, Neisseria, Streptococcus, Actinomyces, and Porphyromonas in the nonsmokers. The differences in the phylum Actinobacteria and genus Corynebacterium contributed to various functional differences between smokers and nonsmokers. The difference on oral microbial and composition between E-cigarettes and common tobacco were associated with increased Prevotellaceae and decreased Neisseria. Additionally, smoking cessation could lead to re-establishment of the oral microbiome to that of nonsmokers. Our data demonstrate that E-cigarette smoking had different effects on the structure and composition of the oral microbial community compared to tobacco smoking. However, the short- and long-term impact of E-cigarette smoking on microbiome composition and function needs further exploration.
Experiment 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking behavior smoking,Smoking behavior,smoking behavior
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non Smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Tobacco smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The volunteers who smoked tobacco
- Group 0 sample size Number of subjects in the control (unexposed) group
- 6
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Ion Torrent
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- ANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-20
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: increased abundance in Tobacco smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae | ||
| Porphyromonas |
Revision editor(s): Annabelcute, Aiyshaaaa, Atrayees
Experiment 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- E-cigarette smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- The volunteers who smoked electronic cigarettes.
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: decreased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Neisseria | ||
| Corynebacterium |
Revision editor(s): Aiyshaaaa
Experiment 3
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Tobacco smokers
- Group 0 sample size Number of subjects in the control (unexposed) group
- 14
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: decreased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Neisseria |
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4 (C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: increased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae |
Experiment 5
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Quitting smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Tobacco smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Volunteers who are tobacco smokers
- Group 0 sample size Number of subjects in the control (unexposed) group
- 8
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months.
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4(C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: increased abundance in Tobacco smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Prevotellaceae | ||
| Porphyromonas |
Revision editor(s): Atrayees
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4(C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: decreased abundance in Tobacco smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Corynebacterium |
Revision editor(s): Atrayees
Experiment 6
Reviewed Marked as Reviewed by ChiomaBlessing on 2024-2-21
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- E-cigarette smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Volunteers who smoke E-cigarettes
- Group 1 sample size Number of subjects in the case (exposed) group
- 5
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-21
Source: Figure 4(C-F)
Description: The relative abundances of Neisseria, Prevotellaceae, Corynebacterium, and Porphyromonas in the two groups.
Abundance in Group 1: decreased abundance in E-cigarette smokers
| NCBI | Quality Control | Links |
|---|---|---|
| Corynebacterium |
Revision editor(s): Atrayees
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