Shifts in the Fecal Microbiota Associated with Adenomatous Polyps

Study information

Reviewed Marked as Reviewed by Lwaldron on 2023-3-20

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

27672054

DOI Digital object identifier for electronic documents.

10.1158/1055-9965.EPI-16-0337

URI

Authors

Hale VL, Chen J, Johnson S, Harrington SC, Yab TC, Smyrk TC, Nelson H, Boardman LA, Druliner BR, Levin TR, Rex DK, Ahnen DJ, Lance P, Ahlquist DA, Chia N

Journal

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology

Year

2017

BACKGROUND: Adenomatous polyps are the most common precursor to colorectal cancer, the second leading cause of cancer-related death in the United States. We sought to learn more about early events of carcinogenesis by investigating shifts in the gut microbiota of patients with adenomas. METHODS: We analyzed 16S rRNA gene sequences from the fecal microbiota of patients with adenomas (n = 233) and without (n = 547). RESULTS: Multiple taxa were significantly more abundant in patients with adenomas, including Bilophila, Desulfovibrio, proinflammatory bacteria in the genus Mogibacterium, and multiple Bacteroidetes species. Patients without adenomas had greater abundances of Veillonella, Firmicutes (Order Clostridia), and Actinobacteria (family Bifidobacteriales). Our findings were consistent with previously reported shifts in the gut microbiota of colorectal cancer patients. Importantly, the altered adenoma profile is predicted to increase primary and secondary bile acid production, as well as starch, sucrose, lipid, and phenylpropanoid metabolism. CONCLUSIONS: These data hint that increased sugar, protein, and lipid metabolism along with increased bile acid production could promote a colonic environment that supports the growth of bile-tolerant microbes such as Bilophilia and Desulfovibrio In turn, these microbes may produce genotoxic or inflammatory metabolites such as H2S and secondary bile acids, which could play a role in catalyzing adenoma development and eventually colorectal cancer. IMPACT: This study suggests a plausible biological mechanism to explain the links between shifts in the microbiota and colorectal cancer. This represents a first step toward resolving the complex interactions that shape the adenoma-carcinoma sequence of colorectal cancer and may facilitate personalized therapeutics focused on the microbiota. Cancer Epidemiol Biomarkers Prev; 26(1); 85-94. ©2016 AACR.

Experiment 1

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Curated date: 2022/06/29

Curator: Jeshudy

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Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Colorectal adenoma adenoma of large bowel,adenoma of large intestine,adenoma of the large bowel,adenoma of the large intestine,colorectal adenoma,colorectum adenoma,large bowel adenoma,large intestine adenoma,Colorectal adenoma

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Non-Adenoma

Group 1 name Corresponds to the case (exposed) group for case-control studies: Adenoma

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with colorectal adenoma as identified during a screening colonoscopy. Fecal samples from patients in which at least one adenoma > 1 cm was identified were included in the “adenoma” group.

Group 0 sample size Number of subjects in the control (unexposed) group: 547

Group 1 sample size Number of subjects in the case (exposed) group: 233

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): None specified

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V5

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: Linear Regression

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.2

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Richness Number of species: unchanged

Signature 1

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Curated date: 2022/07/01

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Source: Figure 2B

Description: −log(P value) of these taxa’s differential abundance.

Abundance in Group 1: increased abundance in Adenoma

NCBI	Quality Control	Links
Alcaligenaceae
Bacteroidales
Bacteroidota
Betaproteobacteria
Bilophila
Burkholderiales
Deltaproteobacteria
Mogibacterium
Sutterella

Revision editor(s): Jeshudy, Suwaiba

Signature 2

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Source: Figure 2B

Description: −log(P value) of these taxa’s differential abundance.

Abundance in Group 1: decreased abundance in Adenoma

NCBI	Quality Control	Links
Actinomycetota
Bacilli
Bacillota
Bifidobacteriales
Clostridia
Clostridiaceae
Clostridium
Cyanobacteriota
Haemophilus
Lentisphaeria
Mollicutes
Mycoplasmatota
Pasteurellaceae
Pasteurellales
Veillonella
unclassified Clostridia
unidentified Actinomycete OPB41

Revision editor(s): Jeshudy, Suwaiba