Ethnic variability associating gut and oral microbiome with obesity in children/Experiment 2
From BugSigDB
Reviewed Marked as Reviewed by Folakunmi on 2024-1-31
Curated date: 2022/06/29
Curator: Kaluifeanyi101
Revision editor(s): Kaluifeanyi101, WikiWorks, Folakunmi
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Oral opening Mouth,Oral fissure,Oral orifice,Oral part of face,Oral opening,oral opening
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ethnic group Ethnicity,race,Ethnic group,ethnic group
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Oral microbiota of European American children (EA)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Oral microbiota of African American children (AA)
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Oral microbiota of 30 African American children (18 female, 12 male; age 6 - 10 years)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 30
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- unspecified
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V3-V5
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- PERMANOVA
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- .05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, body mass index, household income, education level, sex
Alpha Diversity
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 2C
Description: Genus-level differentially abundant taxa in the oral microbiota comparison between AA and EA groups at a 10% false discovery rate
Abundance in Group 1: increased abundance in Oral microbiota of African American children (AA)
| NCBI | Quality Control | Links |
|---|---|---|
| Streptococcus |
Revision editor(s): Kaluifeanyi101, Folakunmi
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-30
Source: Figure 2C
Description: Genus-level differentially abundant taxa in the oral microbiota comparison between AA and EA groups at a 10% false discovery rate
Abundance in Group 1: decreased abundance in Oral microbiota of African American children (AA)
| NCBI | Quality Control | Links |
|---|---|---|
| Butyrivibrio | ||
| Capnocytophaga | ||
| Fusobacterium | ||
| Haemophilus | ||
| Prevotella |
Revision editor(s): Kaluifeanyi101, Folakunmi
