Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa/Experiment 1
Curated date: 2022/07/17
Curator: Kaluifeanyi101
Revision editor(s): Kaluifeanyi101, Atrayees, ChiomaBlessing
Subjects
- Location of subjects
- Burkina Faso
- Italy
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Ethnic group , Place of residence measurement Ethnicity,race,Ethnic group,ethnic group,Place of residence measurement,place of residence measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- European Children in Italy
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Rural African Children in Burkina Faso
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 14 healthy children (nine male and six female) living in the rural village of Boulpon district of Nanoro, Boulkiemde province, Burkina Faso.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V5-V6
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Kruskall-Wallis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Richness Number of species
- increased
Signature 1
Curated date: 2022/07/17
Curator: Kaluifeanyi101
Revision editor(s): Kaluifeanyi101, Atrayees, ChiomaBlessing
Source: Supporting Info. Table S5 + Fig. S2
Description: Report of the species assignment for BF and EU populations relative to the most abundant bacterial genera found by the RDP classifier
Abundance in Group 1: increased abundance in Rural African Children in Burkina Faso
Revision editor(s): Kaluifeanyi101, Atrayees, ChiomaBlessing
Signature 2
Source: Supporting Info. Table S5 + Fig. S2
Description: Report of the species assignment for BF and EU populations relative to the most abundant bacterial genera found by the RDP classifier
Abundance in Group 1: decreased abundance in Rural African Children in Burkina Faso
Revision editor(s): Kaluifeanyi101, ChiomaBlessing
