Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa/Experiment 2

From BugSigDB


Reviewed Marked as Reviewed by ChiomaBlessing on 2024-1-22

Curated date: 2023/07/11

Curator: Atrayees

Revision editor(s): Atrayees, ChiomaBlessing

Subjects

Location of subjects
Burkina Faso
Italy
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Ethnic group , Place of residence measurement Ethnicity,race,Ethnic group,ethnic group,Place of residence measurement,place of residence measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
European Children in Italy
Group 1 name Corresponds to the case (exposed) group for case-control studies
Rural African Children in Burkina Faso
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
14 healthy children (nine male and six female) living in the rural village of Boulpon district of Nanoro, Boulkiemde province, Burkina Faso.
Group 0 sample size Number of subjects in the control (unexposed) group
15
Group 1 sample size Number of subjects in the case (exposed) group
14
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
6 months

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V5-V6
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2024-1-22

Curated date: 2023/07/11

Curator: Atrayees

Revision editor(s): Atrayees, ChiomaBlessing

Source: Figure 3B

Description: Number of sequences relative to principal Enterobacteriaceae genera, in BF and EU children microbiota.

Abundance in Group 1: decreased abundance in Rural African Children in Burkina Faso

NCBI Quality ControlLinks
Escherichia
Shigella

Revision editor(s): Atrayees, ChiomaBlessing