Types of tobacco consumption and the oral microbiome in the United Arab Emirates Healthy Future (UAEHFS) Pilot Study

From BugSigDB
Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Vallès Y, Inman CK, Peters BA, Ali R, Wareth LA, Abdulle A, Alsafar H, Anouti FA, Dhaheri AA, Galani D, Haji M, Hamiz AA, Hosani AA, Houqani MA, Junaibi AA, Kazim M, Kirchhoff T, Mahmeed WA, Maskari FA, Alnaeemi A, Oumeziane N, Ramasamy R, Schmidt AM, Weitzman M, Zaabi EA, Sherman S, Hayes RB, Ahn J
Journal
Scientific reports
Year
2018
Cigarette smoking alters the oral microbiome; however, the effect of alternative tobacco products remains unclear. Middle Eastern tobacco products like dokha and shisha, are becoming globally widespread. We tested for the first time in a Middle Eastern population the hypothesis that different tobacco products impact the oral microbiome. The oral microbiome of 330 subjects from the United Arab Emirates Healthy Future Study was assessed by amplifying the bacterial 16S rRNA gene from mouthwash samples. Tobacco consumption was assessed using a structured questionnaire and further validated by urine cotinine levels. Oral microbiome overall structure and specific taxon abundances were compared, using PERMANOVA and DESeq analyses respectively. Our results show that overall microbial composition differs between smokers and nonsmokers (p = 0.0001). Use of cigarettes (p = 0.001) and dokha (p = 0.042) were associated with overall microbiome structure, while shisha use was not (p = 0.62). The abundance of multiple genera were significantly altered (enriched/depleted) in cigarette smokers; however, only Actinobacillus, Porphyromonas, Lautropia and Bifidobacterium abundances were significantly changed in dokha users whereas no genera were significantly altered in shisha smokers. For the first time, we show that smoking dokha is associated to oral microbiome dysbiosis, suggesting that it could have similar effects as smoking cigarettes on oral health.

Experiment 1


Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13, Claregrieve1, ChiomaBlessing

Subjects

Location of subjects
United Arab Emirates
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behavior smoking,Smoking behavior,smoking behavior
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Nonsmokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Cigarette Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in the form of cigarettes (CS)
Group 0 sample size Number of subjects in the control (unexposed) group
225
Group 1 sample size Number of subjects in the case (exposed) group
33
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
None

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
raw counts
Statistical test
DESeq2
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Table 2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: increased abundance in Cigarette Smokers

NCBI Quality ControlLinks
Atopobium
Bifidobacterium
Campylobacter
Dialister
Enhydrobacter
Megasphaera
Mycoplasma
Paludibacter
Prevotella
Treponema
uncultured Synergistes sp.

Revision editor(s): Adi13, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13

Source: Table 2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: decreased abundance in Cigarette Smokers

NCBI Quality ControlLinks
Porphyromonas
Capnocytophaga
Gemella
Vagococcus
Peptostreptococcus
Fusobacterium
Leptotrichia
Lautropia
Eikenella
Neisseria
Cardiobacterium
Actinobacillus
Aggregatibacter
Haemophilus

Revision editor(s): Adi13

Experiment 2


Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13, Claregrieve1, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Dokha Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in the form of dokha (DS)
Group 1 sample size Number of subjects in the case (exposed) group
16

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/18

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Supplemental. Table S2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: increased abundance in Dokha Smokers

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): Adi13, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/18

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Supplemental. Table S2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: decreased abundance in Dokha Smokers

NCBI Quality ControlLinks
Lautropia
Porphyromonas

Revision editor(s): Adi13, ChiomaBlessing

Experiment 3


Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13, Claregrieve1, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Shisha Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in the form of shisha (SS)
Group 1 sample size Number of subjects in the case (exposed) group
15

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/18

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Supplemental. Table S2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: decreased abundance in Shisha Smokers

NCBI Quality ControlLinks
Candidatus Gracilibacteria bacterium GN02-872
Cyanobacteriota
candidate division SR1 bacterium

Revision editor(s): Adi13, ChiomaBlessing

Experiment 4


Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/17

Curator: Adi13

Revision editor(s): Adi13, Claregrieve1, ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Mutiple Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in multiple forms out of cigarettes (CS), dokha (DS) and shisha (SS)
Group 1 sample size Number of subjects in the case (exposed) group
41

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/18

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Supplemental. Table S2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: increased abundance in Mutiple Smokers

NCBI Quality ControlLinks
Campylobacter
Enhydrobacter
Mycoplasma
Paludibacter
Treponema
uncultured Synergistes sp.

Revision editor(s): Adi13, ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2023-5-4

Curated date: 2023/03/18

Curator: Adi13

Revision editor(s): Adi13, ChiomaBlessing

Source: Supplemental. Table S2

Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers

Abundance in Group 1: decreased abundance in Mutiple Smokers

NCBI Quality ControlLinks
Actinobacillus
Eikenella
Gemella
Haemophilus
Lautropia
Neisseria
Vagococcus

Revision editor(s): Adi13, ChiomaBlessing

Experiment 5


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/05

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Cotinine measurement Cotinine measurement,cotinine measurement
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Cotinine negative
Group 1 name Corresponds to the case (exposed) group for case-control studies
Cotinine positive
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Subjects with positive cotinine result
Group 1 sample size Number of subjects in the case (exposed) group
22

Lab analysis

Statistical Analysis

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/05

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Supplemental. Table S4

Description: Differentially abundant taxa at all taxonomical levels in the contrasts between cotinine positive versus cotinine negative.

Abundance in Group 1: decreased abundance in Cotinine positive

NCBI Quality ControlLinks
Aggregatibacter
Capnocytophaga
Cardiobacterium
Eikenella
Fusobacterium
Gemella
Haemophilus
Lautropia
Neisseria
Peptococcus
Peptostreptococcus
Porphyromonas
Rothia
Weeksellaceae
Cyanobacteriota
Candidatus Gracilibacteria bacterium GN02-872
Pseudomonadota
candidate division SR1 bacterium

Revision editor(s): ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/05

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Supplemental. Table S4

Description: Differentially abundant taxa at all taxonomical levels in the contrasts between cotinine positive versus cotinine negative.

Abundance in Group 1: increased abundance in Cotinine positive

NCBI Quality ControlLinks
Bifidobacterium
Campylobacter
Desulfobulbus
Filifactor
Megasphaera
Mycoplasma
Paludibacter
Treponema
uncultured Synergistes sp.
unclassified Paraprevotella
Spirochaetia

Revision editor(s): ChiomaBlessing

Experiment 6


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/10

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Smoking behavior smoking,Smoking behavior,smoking behavior
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-smokers
Group 1 name Corresponds to the case (exposed) group for case-control studies
Smokers
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco (in any form; cigarettes, dokha, shisha)
Group 1 sample size Number of subjects in the case (exposed) group
105

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased
Simpson Estimator of species richness and species evenness: more weight on species evenness
increased
Richness Number of species
increased

Experiment 7


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/10

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Dokha
Group 1 name Corresponds to the case (exposed) group for case-control studies
CIigarettes
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in the form of cigarettes (CS)
Group 0 sample size Number of subjects in the control (unexposed) group
16
Group 1 sample size Number of subjects in the case (exposed) group
33

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Experiment 8


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/10

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
Shisha
Group 0 sample size Number of subjects in the control (unexposed) group
15

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/11

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Supplemental. Table S3

Description: Differentially abundant taxa at all taxonomical levels between Cigarettes and Shisha

Abundance in Group 1: decreased abundance in CIigarettes

NCBI Quality ControlLinks
Actinobacillus
Butyrivibrio
Capnocytophaga
Comamonadaceae
Fusobacteriia
Gemellaceae
Weeksellaceae

Revision editor(s): ChiomaBlessing

Signature 2

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/11

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Supplemental. Table S3

Description: Differentially abundant taxa at all taxonomical levels between Cigarettes and Shisha

Abundance in Group 1: increased abundance in CIigarettes

NCBI Quality ControlLinks
Paludibacter
Treponema
uncultured Synergistes sp.
Mycoplasma

Revision editor(s): ChiomaBlessing

Experiment 9


Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/10

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Differences from previous experiment shown

Subjects

Group 1 name Corresponds to the case (exposed) group for case-control studies
Dokha
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
People consuming tobacco in the form of Dokha
Group 1 sample size Number of subjects in the case (exposed) group
16

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by ChiomaBlessing on 2023-12-11

Curated date: 2023/12/11

Curator: ChiomaBlessing

Revision editor(s): ChiomaBlessing

Source: Supplemental. Table S3

Description: Differentially abundant taxa at all taxonomical levels between Dokha and Shisha

Abundance in Group 1: decreased abundance in Dokha

NCBI Quality ControlLinks
Actinobacillus
Weeksellaceae
Gemellaceae

Revision editor(s): ChiomaBlessing