Types of tobacco consumption and the oral microbiome in the United Arab Emirates Healthy Future (UAEHFS) Pilot Study
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Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
Experiment 1
Subjects
- Location of subjects
- United Arab Emirates
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking behavior smoking,Smoking behavior,smoking behavior
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Nonsmokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Cigarette Smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in the form of cigarettes (CS)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 225
- Group 1 sample size Number of subjects in the case (exposed) group
- 33
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- None
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Signature 1
Source: Table 2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: increased abundance in Cigarette Smokers
NCBI | Quality Control | Links |
---|---|---|
Atopobium | ||
Bifidobacterium | ||
Campylobacter | ||
Dialister | ||
Enhydrobacter | ||
Megasphaera | ||
Mycoplasma | ||
Paludibacter | ||
Prevotella | ||
Treponema | ||
uncultured Synergistes sp. |
Revision editor(s): Adi13, ChiomaBlessing
Signature 2
Source: Table 2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: decreased abundance in Cigarette Smokers
Revision editor(s): Adi13
Experiment 2
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Dokha Smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in the form of dokha (DS)
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Supplemental. Table S2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: increased abundance in Dokha Smokers
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium |
Revision editor(s): Adi13, ChiomaBlessing
Signature 2
Source: Supplemental. Table S2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: decreased abundance in Dokha Smokers
NCBI | Quality Control | Links |
---|---|---|
Lautropia | ||
Porphyromonas |
Revision editor(s): Adi13, ChiomaBlessing
Experiment 3
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Shisha Smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in the form of shisha (SS)
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 2
Source: Supplemental. Table S2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: decreased abundance in Shisha Smokers
NCBI | Quality Control | Links |
---|---|---|
Candidatus Gracilibacteria bacterium GN02-872 | ||
Cyanobacteriota | ||
candidate division SR1 bacterium |
Revision editor(s): Adi13, ChiomaBlessing
Experiment 4
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Mutiple Smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in multiple forms out of cigarettes (CS), dokha (DS) and shisha (SS)
- Group 1 sample size Number of subjects in the case (exposed) group
- 41
Lab analysis
Statistical Analysis
Signature 1
Source: Supplemental. Table S2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: increased abundance in Mutiple Smokers
NCBI | Quality Control | Links |
---|---|---|
Campylobacter | ||
Enhydrobacter | ||
Mycoplasma | ||
Paludibacter | ||
Treponema | ||
uncultured Synergistes sp. |
Revision editor(s): Adi13, ChiomaBlessing
Signature 2
Source: Supplemental. Table S2
Description: Differentially abundant taxa at selected taxonomical levels by type of tobacco use, compared to nonsmokers
Abundance in Group 1: decreased abundance in Mutiple Smokers
NCBI | Quality Control | Links |
---|---|---|
Actinobacillus | ||
Eikenella | ||
Gemella | ||
Haemophilus | ||
Lautropia | ||
Neisseria | ||
Vagococcus |
Revision editor(s): Adi13, ChiomaBlessing
Experiment 5
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Cotinine measurement Cotinine measurement,cotinine measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Cotinine negative
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Cotinine positive
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Subjects with positive cotinine result
- Group 1 sample size Number of subjects in the case (exposed) group
- 22
Lab analysis
Statistical Analysis
Signature 1
Source: Supplemental. Table S4
Description: Differentially abundant taxa at all taxonomical levels in the contrasts between cotinine positive versus cotinine negative.
Abundance in Group 1: decreased abundance in Cotinine positive
Revision editor(s): ChiomaBlessing
Signature 2
Source: Supplemental. Table S4
Description: Differentially abundant taxa at all taxonomical levels in the contrasts between cotinine positive versus cotinine negative.
Abundance in Group 1: increased abundance in Cotinine positive
NCBI | Quality Control | Links |
---|---|---|
Bifidobacterium | ||
Campylobacter | ||
Desulfobulbus | ||
Filifactor | ||
Megasphaera | ||
Mycoplasma | ||
Paludibacter | ||
Treponema | ||
uncultured Synergistes sp. | ||
unclassified Paraprevotella | ||
Spirochaetia |
Revision editor(s): ChiomaBlessing
Experiment 6
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Smoking behavior smoking,Smoking behavior,smoking behavior
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Non-smokers
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Smokers
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco (in any form; cigarettes, dokha, shisha)
- Group 1 sample size Number of subjects in the case (exposed) group
- 105
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
- Richness Number of species
- increased
Experiment 7
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Dokha
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- CIigarettes
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in the form of cigarettes (CS)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 33
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Experiment 8
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Shisha
- Group 0 sample size Number of subjects in the control (unexposed) group
- 15
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Supplemental. Table S3
Description: Differentially abundant taxa at all taxonomical levels between Cigarettes and Shisha
Abundance in Group 1: decreased abundance in CIigarettes
NCBI | Quality Control | Links |
---|---|---|
Actinobacillus | ||
Butyrivibrio | ||
Capnocytophaga | ||
Comamonadaceae | ||
Fusobacteriia | ||
Gemellaceae | ||
Weeksellaceae |
Revision editor(s): ChiomaBlessing
Signature 2
Source: Supplemental. Table S3
Description: Differentially abundant taxa at all taxonomical levels between Cigarettes and Shisha
Abundance in Group 1: increased abundance in CIigarettes
NCBI | Quality Control | Links |
---|---|---|
Paludibacter | ||
Treponema | ||
uncultured Synergistes sp. | ||
Mycoplasma |
Revision editor(s): ChiomaBlessing
Experiment 9
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Dokha
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- People consuming tobacco in the form of Dokha
- Group 1 sample size Number of subjects in the case (exposed) group
- 16
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
Signature 1
Source: Supplemental. Table S3
Description: Differentially abundant taxa at all taxonomical levels between Dokha and Shisha
Abundance in Group 1: decreased abundance in Dokha
NCBI | Quality Control | Links |
---|---|---|
Actinobacillus | ||
Weeksellaceae | ||
Gemellaceae |
Revision editor(s): ChiomaBlessing