A taxonomic signature of obesity in a large study of American adults
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Peters BA, Shapiro JA, Church TR, Miller G, Trinh-Shevrin C, Yuen E, Friedlander C, Hayes RB, Ahn J
Journal
Scientific reports
Year
2018
Animal models suggest that gut microbiota contribute to obesity; however, a consistent taxonomic signature of obesity has yet to be identified in humans. We examined whether a taxonomic signature of obesity is present across two independent study populations. We assessed gut microbiome from stool for 599 adults, by 16S rRNA gene sequencing. We compared gut microbiome diversity, overall composition, and individual taxon abundance for obese (BMI ≥ 30 kg/m2), overweight (25 ≤ BMI < 30), and healthy-weight participants (18.5 ≤ BMI < 25). We found that gut species richness was reduced (p = 0.04), and overall composition altered (p = 0.04), in obese (but not overweight) compared to healthy-weight participants. Obesity was characterized by increased abundance of class Bacilli and its families Streptococcaceae and Lactobacillaceae, and decreased abundance of several groups within class Clostridia, including Christensenellaceae, Clostridiaceae, and Dehalobacteriaceae (q < 0.05). These findings were consistent across two independent study populations. When random forest models were trained on one population and tested on the other as well as a previously published dataset, accuracy of obesity prediction was good (~70%). Our large study identified a strong and consistent taxonomic signature of obesity. Though our study is cross-sectional and causality cannot be determined, identification of microbes associated with obesity can potentially provide targets for obesity prevention and treatment.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal weight
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy-weight was defined as BMI ≥ 18.5 and <25 kg/m2, overweight as BMI ≥ 25 and <30 kg/m2, and obese as BMI ≥ 30 kg/m2
- Group 0 sample size Number of subjects in the control (unexposed) group
- 211
- Group 1 sample size Number of subjects in the case (exposed) group
- 142
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- long term use in NYU study. NA for CDC study
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Statistical test
- DESeq2
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment
- age, sex, polyp
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 4
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in obese
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 4
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: decreased abundance in obese
Experiment 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight
- Group 1 sample size Number of subjects in the case (exposed) group
- 246
Lab analysis
Statistical Analysis
Alpha Diversity
- Shannon Estimator of species richness and species evenness: more weight on species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 4
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in overweight
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 4
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: decreased abundance in overweight
| NCBI | Quality Control | Links |
|---|---|---|
| Christensenellaceae | ||
| Clostridiaceae | ||
| Dehalobacterium | ||
| Erysipelotrichaceae | ||
| Eubacteriales | ||
| Oscillospira | ||
| Oscillospiraceae | ||
| Parabacteroides |
Experiment 3
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal weight men
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese men
- Group 0 sample size Number of subjects in the control (unexposed) group
- 80
- Group 1 sample size Number of subjects in the case (exposed) group
- 70
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in obese men
| NCBI | Quality Control | Links |
|---|---|---|
| Parabacteroides distasonis | ||
| Bacilli | ||
| Lactobacillales | ||
| Lactobacillaceae | ||
| Lactobacillus | ||
| Streptococcus | ||
| Blautia | ||
| Oscillospiraceae | ||
| Veillonellaceae | ||
| Pseudomonadota | ||
| Gammaproteobacteria |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: decreased abundance in obese men
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridiaceae | ||
| Corynebacteriaceae | ||
| Corynebacterium | ||
| Dehalobacterium | ||
| Ruminococcus | ||
| Tissierellaceae |
Experiment 4
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight men
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy-weight was defined as BMI ≥ 18.5 and <25 kg/m2, overweight as BMI ≥ 25 and <30 kg/m2, and obese as BMI ≥ 30 kg/m3
- Group 1 sample size Number of subjects in the case (exposed) group
- 171
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in overweight men
| NCBI | Quality Control | Links |
|---|---|---|
| Bacteroides | ||
| Bacilli | ||
| Lactobacillales | ||
| Lactobacillaceae | ||
| Lactobacillus | ||
| Streptococcus | ||
| Blautia | ||
| Coprococcus | ||
| Lachnobacterium |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: decreased abundance in overweight men
Experiment 5
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- normal weight women
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese women
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy-weight was defined as BMI ≥ 18.5 and <25 kg/m2, overweight as BMI ≥ 25 and <30 kg/m2, and obese as BMI ≥ 30 kg/m5
- Group 0 sample size Number of subjects in the control (unexposed) group
- 131
- Group 1 sample size Number of subjects in the case (exposed) group
- 72
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in obese women
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: decreased abundance in obese women
| NCBI | Quality Control | Links |
|---|---|---|
| Christensenellaceae | ||
| Clostridiaceae | ||
| Dehalobacterium | ||
| Clostridia | ||
| Clostridiales bacterium |
Experiment 6
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- overweight women
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Healthy-weight was defined as BMI ≥ 18.5 and <25 kg/m2, overweight as BMI ≥ 25 and <30 kg/m2, and obese as BMI ≥ 30 kg/m7
- Group 1 sample size Number of subjects in the case (exposed) group
- 75
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Supplemental Table 5
Description: Taxonomic sginature of obesity in a large study of American adults
Abundance in Group 1: increased abundance in overweight women
Revision editor(s): WikiWorks
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