Duodenal and rectal mucosal microbiota related to small intestinal bacterial overgrowth in diarrhea-predominant irritable bowel syndrome/Experiment 4

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Reviewed Marked as Reviewed by Folakunmi on 2024-2-8

Curated date: 2022/07/30

Curator: Jeshudy

Revision editor(s): WikiWorks, Jeshudy, Atrayees, Folakunmi

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Duodenum Proximal intestine,Upper intestine,Duodenum,duodenum

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Small intestine enteropathy small bowel enteropathy,Small intestine enteropathy,small intestine enteropathy

Group 0 name Corresponds to the control (unexposed) group for case-control studies

HC.sibo-

Group 1 name Corresponds to the case (exposed) group for case-control studies

HC.sibo+

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

duodenal fluid samples of healthy controls (with no IBS) with SIBO

Group 0 sample size Number of subjects in the control (unexposed) group

12

Group 1 sample size Number of subjects in the case (exposed) group

7

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)

3 months; also includes probiotics and proton-pump inhibitors

Lab analysis

Sequencing type

16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

V1-V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Chi-Square

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment

age, body mass index, sex

Alpha Diversity

Chao1 Abundance-based estimator of species richness

unchanged

Signature 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-2-8

Curated date: 2022/07/30

Curator: Jeshudy

Revision editor(s): Jeshudy

Source: Table S1

Description: Comparisons of microbial composition at the phylum level between SIBO+ and SIBO- IBS-D patients and healthy individuals.

Abundance in Group 1: increased abundance in HC.sibo+

NCBI	Quality Control	Links
Bacteroidota

Revision editor(s): Jeshudy