Effects of prebiotics on immunologic indicators and intestinal microbiota structure in perioperative colorectal cancer patients/Experiment 1

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Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/22

Curated date: 2022/09/03

Curator: Mary Bearkland

Revision editor(s): WikiWorks, LGeistlinger, Claregrieve1, Mary Bearkland, Peace Sandy

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Nutraceutical , Colorectal cancer Dietary Supplement,Food Supplementation,Nutritional supplement,Nutraceutical,nutraceutical,cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
colorectal cancer patients who didn't take prebiotics (preoperative fecal sample)
Group 1 name Corresponds to the case (exposed) group for case-control studies
colorectal cancer patients who took prebiotics (preoperative fecal sample)
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients with radiologic and pathologic diagnosis of CRC (preoperatively). Patients in the intervention group (prebiotic group) received a daily oral dose of 30 g prebiotic supplement (Hangzhou Niuqu Biotech Co., Hainengbo, China) containing fructooligosaccharide (25%), xylooligosaccharide (25%), polydextrose (25%), and resistant dextrin (25%) for 7 d from hospitalization to the day before the operation.
Group 0 sample size Number of subjects in the control (unexposed) group
20
Group 1 sample size Number of subjects in the case (exposed) group
18
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
no treatment with antibiotics within 6 mo before surgery

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V3-V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness
increased

Signature 1

Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/22

Curated date: 2022/09/03

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Claregrieve1

Source: Table 4; Table 3

Description: Differential microbial abundance between patients who took prebiotics and patients who did not

Abundance in Group 1: decreased abundance in colorectal cancer patients who took prebiotics (preoperative fecal sample)

NCBI Quality ControlLinks
Bacteroides
Enterococcus

Revision editor(s): Mary Bearkland, Claregrieve1

Signature 2

Reviewed Marked as Reviewed by Claregrieve1 on 2022/12/22

Curated date: 2022/09/03

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Claregrieve1

Source: Table 4; Table 3

Description: Differential microbial abundance between patients who took prebiotics and patients who did not

Abundance in Group 1: increased abundance in colorectal cancer patients who took prebiotics (preoperative fecal sample)

NCBI Quality ControlLinks
Bifidobacterium

Revision editor(s): Mary Bearkland, Claregrieve1