Gut microbiome affects the response to anti-PD-1 immunotherapy in patients with hepatocellular carcinoma/Experiment 1

From BugSigDB


Reviewed Marked as Reviewed by Fatima on 2022/09/7

Curated date: 2022/09/03

Curator: Sharmilac

Revision editor(s): Sharmilac, Fatima, Peace Sandy

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Response to immunochemotherapy Response to immunochemotherapy,response to immunochemotherapy
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Non-responders
Group 1 name Corresponds to the case (exposed) group for case-control studies
Responders
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Responders (R, n = 3) were defined by radiographic evidence as complete or partial response, or stable disease lasting for at least six months.
Group 0 sample size Number of subjects in the control (unexposed) group
5
Group 1 sample size Number of subjects in the case (exposed) group
3

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
BGISEQ-500 Sequencing

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Fatima on 2022/10/27

Curated date: 2022/09/03

Curator: Sharmilac

Revision editor(s): Sharmilac, Fatima

Source: text, Figure 2A, Figure S4

Description: Meta-analysis of the bacteria significantly enriched in R and NR. a Heatmap showing the relative abundance of R-enriched and NR-enriched bacterial species, as identified by LEfSe. Differentially abundant genera (a) and species (b) between R and NR, identified by LEfSe.

Abundance in Group 1: increased abundance in Responders

NCBI Quality ControlLinks
Akkermansia muciniphila
Anaerobutyricum hallii
Anaerotruncus colihominis
Bacteroides cellulosilyticus
Bifidobacterium dentium
Blautia obeum
Candidatus Liberibacter brunswickensis
Coprococcus comes
Dialister invisus
Dorea formicigenerans
Fusobacterium ulcerans
Lachnospiraceae bacterium 7_1_58FAA
Lactobacillus gasseri
Limosilactobacillus mucosae
Limosilactobacillus oris
Limosilactobacillus vaginalis
Megasphaera micronuciformis
Ruminococcus bromii
Salinibacter
Streptococcus thermophilus
Subdoligranulum
Lactobacillus
Bifidobacterium
unclassified Subdoligranulum
Ruminococcus
Akkermansia
Dialister
Scardovia

Revision editor(s): Sharmilac, Fatima

Signature 2

Reviewed Marked as Reviewed by Fatima on 2022/10/27

Curated date: 2022/09/03

Curator: Sharmilac

Revision editor(s): Sharmilac, Fatima, Aiyshaaaa

Source: text, Figure 2A

Description: Meta-analysis of the bacteria significantly enriched in R and NR. a Heatmap showing the relative abundance of R-enriched and NR-enriched bacterial species, as identified by LEfSe.

Abundance in Group 1: decreased abundance in Responders

NCBI Quality ControlLinks
Aggregatibacter aphrophilus
Bacteroides eggerthii
Bacteroides fluxus
Bacteroides nordii
Bacteroides uniformis
Bartonella
Bifidobacterium adolescentis
Bifidobacterium bifidum
Bordetella
Escherichia albertii
Fusobacterium varium
Haemophilus pittmaniae
Klebsiella aerogenes
Ligilactobacillus salivarius
Megasphaera elsdenii
Turicibacter
Veillonella atypica
Veillonella dispar
unclassified Propionibacteriaceae
Cellvibrio

Revision editor(s): Sharmilac, Fatima, Aiyshaaaa