Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway/Experiment 4

From BugSigDB


Needs review

Curated date: 2022/09/18

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Atrayees, Victoria

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Antimicrobial agent antibiotic,antibiotics,Antibiotika,Antibiotikum,antibiotique,antimicrobial,antimicrobial agents,microbicide,microbicides,Antimicrobial agent,antimicrobial agent
Group 0 name Corresponds to the control (unexposed) group for case-control studies
control
Group 1 name Corresponds to the case (exposed) group for case-control studies
Coli
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Group 1 mice were treated with colistin (2 mg/ml) in sterile drinking water (Coli group). Mice (in all groups) were then subcutaneously injected into the right axillary with 0.25 live CT26 tumor cells.
Group 0 sample size Number of subjects in the control (unexposed) group
6
Group 1 sample size Number of subjects in the case (exposed) group
7

Lab analysis

Sequencing type
WMS
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
Not specified
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
Mann-Whitney (Wilcoxon)
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05


Signature 1

Reviewed Marked as Reviewed by Atrayees on 2023-7-14

Curated date: 2022/09/18

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland

Source: supp fig 2

Description: Bar plot of compositional differences at species level in the Control vs. Coli

Abundance in Group 1: increased abundance in Coli

NCBI Quality ControlLinks
Bacteroidaceae bacterium
Bacteroides ovatus
Bacteroides xylanisolvens
Parabacteroides sp.
unclassified Bacteroidales
uncultured prokaryote

Revision editor(s): Mary Bearkland

Signature 2

Reviewed Marked as Reviewed by Atrayees on 2023-7-14

Curated date: 2022/09/18

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Merit, Atrayees

Source: supp fig 2

Description: Bar plot of compositional differences at species level in the Control vs. Coli

Abundance in Group 1: decreased abundance in Coli

NCBI Quality ControlLinks
Bacteroides oleiciplenus
Bacteroides sp. CAG:530
Bacteroides sp. CAG:633
Parabacteroides goldsteinii
Phocaeicola massiliensis
Phocaeicola sartorii
uncultured bacterium
Bacteroides sp. CAG:462

Revision editor(s): Mary Bearkland, Merit, Atrayees