Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway/Experiment 6

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Curated date: 2022/09/21

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland

Subjects

Location of subjects

China

Host species Species from which microbiome was sampled. Contact us to have more species added.

Mus musculus

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Antimicrobial agent antibiotic,antibiotics,Antibiotika,Antibiotikum,antibiotique,antimicrobial,antimicrobial agents,microbicide,microbicides,Antimicrobial agent,antimicrobial agent

Group 0 name Corresponds to the control (unexposed) group for case-control studies

coli

Group 1 name Corresponds to the case (exposed) group for case-control studies

Vanc

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Group 1 mice were treated with vancomycin alone (0.25 mg/ml) in sterile drinking water (Vanc group) Mice (in all groups) were then subcutaneously injected into the right axillary with 0.25 live CT26 tumor cells.

Group 0 sample size Number of subjects in the control (unexposed) group

7

Group 1 sample size Number of subjects in the case (exposed) group

7

Lab analysis

Sequencing type

WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Statistical test

ANOVA

Signature 1

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Reviewed Marked as Reviewed by Atrayees on 2023-7-14

Curated date: 2022/09/21

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland

Source: supp fig 2

Description: Bar plot of compositional differences at species level in the Vanc vs.Coli groups of mice before PD-1 antibody treatment by one-way ANOVA.

Abundance in Group 1: increased abundance in Vanc

NCBI	Quality Control	Links
Prevotella sp. CAG:485
Parabacteroides distasonis
Akkermansia muciniphila
unclassified Bacteroidales
Bacteroides uniformis
unclassified Parabacteroides
Akkermansia muciniphila CAG:154
Parasutterella excrementihominis

Revision editor(s): Mary Bearkland

Signature 2

EditHistoryDelete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-7-14

Curated date: 2022/09/21

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland

Source: supp fig 2

Description: Bar plot of compositional differences at species level in the Vanc vs.Coli groups of mice before PD-1 antibody treatment by one-way ANOVA.

Abundance in Group 1: decreased abundance in Vanc

NCBI	Quality Control	Links
Bacteroides sp. CAG:927
Prevotella sp. CAG:1031
Prevotella sp. CAG:873
Prevotella sp. CAG:279
Phocaeicola vulgatus
Lachnospiraceae bacterium A4
Firmicutes bacterium ASF500

Revision editor(s): Mary Bearkland