Pyrosequencing study of fecal microflora of autistic and control children

From BugSigDB
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 9 February 2021
study design
case-control
Citation
PMID
URI
Authors
Finegold SM, Dowd SE, Gontcharova V, Liu C, Henley KE, Wolcott RD, Youn E, Summanen PH, Granpeesheh D, Dixon D, Liu M, Molitoris DR, Green JA
Journal
Anaerobe
Year
2010
There is evidence of genetic predisposition to autism, but the percent of autistic subjects with this background is unknown. It is clear that other factors, such as environmental influences, may play a role in this disease. In the present study, we have examined the fecal microbial flora of 33 subjects with various severities of autism with gastrointestinal symptoms, 7 siblings not showing autistic symptoms (sibling controls) and eight non-sibling control subjects, using the bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP) procedure. The results provide us with information on the microflora of stools of young children and a compelling picture of unique fecal microflora of children with autism with gastrointestinal symptomatology. Differences based upon maximum observed and maximum predicted operational taxonomic units were statistically significant when comparing autistic and control subjects with p-values ranging from <0.001 to 0.009 using both parametric and non-parametric estimators. At the phylum level, Bacteroidetes and Firmicutes showed the most difference between groups of varying severities of autism. Bacteroidetes was found at high levels in the severely autistic group, while Firmicutes were more predominant in the control group. Smaller, but significant, differences also occurred in the Actinobacterium and Proteobacterium phyla. Desulfovibrio species and Bacteroides vulgatus are present in significantly higher numbers in stools of severely autistic children than in controls. If the unique microbial flora is found to be a causative or consequent factor in this type of autism, it may have implications with regard to a specific diagnostic test, its epidemiology, and for treatment and prevention.

Experiment 1


Reviewed Marked as Reviewed by Fatima on 29 June 2021

Curated date: 2021/01/10

Curator: WikiWorks743

Revision editor(s): WikiWorks753, WikiWorks743, Rimsha

Subjects

Location of subjects Country from which study subjects were recruited
United States of America
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
autism autism,autism (disease),autism spectrum disorder,Autism, Early Infantile,Autism, Infantile,autism, susceptiblity to,Autisms,Autistic Disorder,autistic disorder,autistic disorder of childhood onset,Autistic disorder of childhood onset (disorder),childhood autism,Disorder, Autistic,Disorders, Autistic,Early Infantile Autism,Infantile Autism,infantile autism,Infantile autism (disorder),Infantile Autism, Early,Infantile psychosis (disorder),Kanner Syndrome,Kanner's Syndrome,Kanner's syndrome,Kanners Syndrome,Syndrome, Kanner's
Group 0 name Corresponds to the control (unexposed) group for case-control studies
non-sibling control
Group 1 name Corresponds to the case (exposed) group for case-control studies
severely autistic
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Cases were diagnosed with autistic spectrum disorder. Moreover, they were evaluated for autism and their diagnoses were validated based on impairment in social skills, impairment in language skills and verbal communication, sensory disturbances, repetitive stereotypical behaviors, and gastrointestinal disturbances.
Group 0 sample size Number of subjects in the control (unexposed) group
8
Group 1 sample size Number of subjects in the case (exposed) group
11
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
within preceding month

Lab analysis

Sequencing type Experimental technique used for quantifying microbial abundance
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Roche454

Statistical Analysis

Statistical test Statistical test or computational tool used for differential abundance testing
T-Test
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No


Alpha Diversity

Chao1 Abundance-based estimator of species richness
increased
Richness Number of species
increased

Signature 1

Reviewed Marked as Reviewed by Shaimaa Elsafoury on 9 February 2021

Curated date: 2018-09-11

Curator: Marianthi Thomatos

Revision editor(s): WikiWorks743

Source: Table 2 + Table 3 + Table 5 + Table 6 + Table 8

Description: Significant fecal microflora of autistic and control children

Abundance in Group 1: increased abundance in severely autistic

NCBI Links
Turicibacter
Bacteroides
Alkaliflexus
Desulfovibrio
Acetanaerobacterium
Parabacteroides
Desulfovibrio piger
Desulfovibrio desulfuricans
Desulfovibrio intestinalis
Phocaeicola vulgatus
Bacteroidota
Proteobacteria

Revision editor(s): WikiWorks743

Signature 2

Reviewed Marked as Reviewed by Fatima on 28 July 2021

Curated date: 2018-09-11

Curator: Marianthi Thomatos

Revision editor(s): Lwaldron, WikiWorks743

Source: Table 2 + Table 3 + Table 5 + Table 6 + Table 8

Description: Significant fecal microflora of autistic and control children

Abundance in Group 1: decreased abundance in severely autistic

NCBI Links
Actinobacteria
Alkaliphilus
Anaerofilum
Anaerovorax
Bifidobacterium
Bifidobacterium angulatum
Bifidobacterium longum subsp. longum
Clostridium
Collinsella
Dialister
Dialister invisus
Ethanoligenens
Firmicutes
Helcococcus
Lactobacillus
Leuconostoc
Pseudoramibacter
Ruminococcus
Streptococcus
Weissella
[Clostridium] leptum

Revision editor(s): Lwaldron, WikiWorks743