Oral status in patients with early rheumatoid arthritis: a prospective, case-control study

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-1-22

study design

cross-sectional observational, not case-control

Citation

PMID PubMed identifier for scientific articles.

24273047

DOI Digital object identifier for electronic documents.

10.1093/rheumatology/ket362

URI

Authors

Wolff B, Berger T, Frese C, Max R, Blank N, Lorenz HM, Wolff D

Journal

Rheumatology (Oxford, England)

Year

2014

Keywords:

case–control studies, oral hygiene, periodontal attachment loss, periodontal index, plaque index, rheumatoid arthritis

OBJECTIVE: Patients with RA suffer from a higher risk of periodontal attachment loss and increased oral inflammation. We hypothesize that there are pathogenetic and immunological interactions between these diseases that go beyond impaired manual dexterity accompanying advanced RA. The primary objective of the present study was to determine whether a loss of alveolar bone can be detected in RA patients during the early course of the disease. METHODS: In this cross-sectional, epidemiological case-control study, 22 patients with early RA (ERA) were compared with 22 matched healthy controls. Oral and periodontal status, clinical activity, and socio-demographic parameters were determined. Oral microbiota were analysed using real-time quantitative PCR specific for leading oral pathogens. RESULTS: More advanced forms of periodontitis were found in ERA patients compared with controls. ERA patients had a greater number of missing teeth [ERA 5.7 (s.d. 5.0), controls 1.9 (s.d. 1.0), P = 0.002], deeper periodontal pockets [clinical attachment level: ERA 3.4 (s.d. 0.5 mm), controls 2.7 (s.d. 0.3 mm), P < 0.000], and greater bleeding on probing [ERA 18.6% (s.d. 9.0%), controls 10.5% (s.d. 5.1%), P = 0.001] despite comparable oral hygiene. Tannerella forsythia (6.77-fold, P = 0.033) subgingivally and Streptococcus anginosus (3.56-fold, P = 0.028) supragingivally were the characteristic pathogens in ERA. CONCLUSION: Increased loss of periodontal attachment and alveolar bone can be detected in patients with ERA, therefore we propose that the consulting rheumatologists inform the patients that they have a higher risk of periodontal disease. It would be beneficial if these patients were referred directly for intensive dental care.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-22

Curated date: 2022/11/02

Curator: Tislam

Revision editor(s): WikiWorks, Tislam, Atrayees, Peace Sandy

Subjects

Location of subjects: Germany

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Subgingival dental plaque Subgingival plaque,Subgingival dental plaque,subgingival dental plaque

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Rheumatoid arthritis Arthritis or polyarthritis, rheumatic,arthritis or polyarthritis, rheumatic,Arthritis, Rheumatoid,arthritis, rheumatoid,atrophic Arthritis,atrophic arthritis,autoimmune arthritis,Chronic rheumatic arthritis,Proliferative arthritis,RA,RA - Rheumatoid arthritis,RhA - Rheumatoid arthritis,Rheumatic gout,rheumatoid arthritis,Rheumatoid arthritis (disorder),Rheumatoid arthritis NOS,Rheumatoid arthritis NOS (disorder),rheumatoid arthritis, susceptibility to,Rheumatoid disease,Rheumatoid arthritis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy Controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Early Rheumatoid Arthritis

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with Early Rheumatoid Arthritis

Group 0 sample size Number of subjects in the control (unexposed) group: 22

Group 1 sample size Number of subjects in the case (exposed) group: 22

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Intake of antibiotics during the past 6 months

Lab analysis

Sequencing type: PCR

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: RT-qPCR

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: Yes

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-22

Curated date: 2022/11/02

Curator: Tislam

Revision editor(s): Tislam, Atrayees, Peace Sandy

Source: Table 3, Table 4, Text

Description: Microbiological data and quantitative real-time PCR measurements of abundance of subgingival microbiota in ERA patients and controls.

Microbiological data and quantitative real-time PCR measurements of abundance of supragingival microbiota in ERA patients and controls

Abundance in Group 1: increased abundance in Early Rheumatoid Arthritis

NCBI	Quality Control	Links
Tannerella forsythia
Streptococcus anginosus

Revision editor(s): Tislam, Atrayees, Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-1-22

Curated date: 2022/11/02

Curator: Tislam

Revision editor(s): Tislam, Peace Sandy

Source: Table 3, Table 4, Text

Description: Microbiological data and quantitative real-time PCR measurements of abundance of subgingival microbiota in ERA patients and controls

Microbiological data and quantitative real-time PCR measurements of abundance of supragingival microbiota in ERA patients and controls

Abundance in Group 1: decreased abundance in Early Rheumatoid Arthritis

NCBI	Quality Control	Links
Actinomyces sp.
Corynebacterium durum
Fusobacteriota
Lactobacillus gasseri
Leptotrichia sp.
Rothia dentocariosa
Streptococcus gordonii
Lactobacillus

Revision editor(s): Tislam, Peace Sandy