Oral Microbiota Identifies Patients in Early Onset Rheumatoid Arthritis

Study information

Needs review

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

34442739

DOI Digital object identifier for electronic documents.

10.3390/microorganisms9081657

URI

Authors

Esberg A, Johansson L, Johansson I, Dahlqvist SR

Journal

Microorganisms

Year

2021

Keywords:

16S rDNA sequencing, oral microbiota, periodontitis, rheumatoid arthritis

Rheumatoid arthritis (RA) is the most common autoimmune inflammatory disease, and single periodontitis-associated bacteria have been suggested in disease manifestation. Here, the oral microbiota was characterized in relation to the early onset of RA (eRA) taking periodontal status into consideration. 16S rRNA gene amplicon sequencing of saliva bacterial DNA from 61 eRA patients without disease-modifying anti-rheumatic drugs and 59 matched controls was performed. Taxonomic classification at 98.5% was conducted against the Human Oral Microbiome Database, microbiota functions were predicted using PICRUSt, and periodontal status linked from the Swedish quality register for clinically assessed caries and periodontitis. The participants were classified into three distinct microbiota-based cluster groups with cluster allocation differences by eRA status. Independently of periodontal status, eRA patients had enriched levels of Prevotella pleuritidis, Treponema denticola, Porphyromonas endodontalis and Filifactor alocis species and in the Porphyromonas and Fusobacterium genera and functions linked to ornithine metabolism, glucosylceramidase, beta-lactamase resistance, biphenyl degradation, fatty acid metabolism and 17-beta-estradiol-17-dehydrogenase metabolism. The results support a deviating oral microbiota composition already in eRA patients compared with healthy controls and highlight a panel of oral bacteria that may be useful in eRA risk assessment in both periodontally healthy and diseased persons.

Experiment 1

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Curated date: 2022/11/02

Curator: Tislam

Revision editor(s): Tislam, WikiWorks

Subjects

Location of subjects: United States of America

Host species Species from which microbiome was sampled (if applicable): Homo sapiens

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: arthritis arthritic joint disease,arthritides,arthritis,inflammation of skeletal joint,inflammatory disorder of joint,skeletal joint inflammation

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy Controls
Group 1 name Corresponds to the case (exposed) group for case-control studies: Early Rheumatoid Arthritis
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Early Rheumatoid Arthritis PATIENTS
Group 0 sample size Number of subjects in the control (unexposed) group: 59
Group 1 sample size Number of subjects in the case (exposed) group: 61
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): 3 months

Not specified

Statistical Analysis

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): .05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2
Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Signature 1

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Source: Figure 2, text

Description: (c) Bar graph showing top 20 bacterial species and smoking status influential in the OPLS-DA model and expressed as Variable Importance in Projection (VIP) values with 95% confidence interval. Red colour represents eRA and blue colour healthy controls. For a full list of VIP values see Table S2.

Abundance in Group 1: decreased abundance in Early Rheumatoid Arthritis

NCBI	Quality Control	Links
Oribacterium sinus
Catonella morbi
Veillonella rogosae
Campylobacter concisus

Revision editor(s): Tislam

Signature 2

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Mark reviewed

Your review change was submitted

Needs review

Curated date: 2022/11/02

Curator: Tislam

Revision editor(s): Tislam

Source: Figure 2, text

Description: (c) Bar graph showing top 20 bacterial species and smoking status influential in the OPLS-DA model and expressed as Variable Importance in Projection (VIP) values with 95% confidence interval. Red colour represents eRA and blue colour healthy controls. For a full list of VIP values see Table S2.

Abundance in Group 1: increased abundance in Early Rheumatoid Arthritis

NCBI	Quality Control	Links
Alloprevotella tannerae
Campylobacter gracilis
Capnocytophaga leadbetteri
Dialister pneumosintes
Filifactor alocis
Porphyromonas endodontalis
Prevotella dentalis
Prevotella intermedia
Hoylesella pleuritidis
Prevotella sp.
Stomatobaculum
Treponema denticola

Revision editor(s): Tislam