Salivary microbiota and inflammation-related proteins in patients with psoriasis
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Belstrøm D, Eiberg JM, Enevold C, Grande MA, Jensen CAJ, Skov L, Hansen PR
Journal
Oral diseases
Year
2020
Keywords:
inflammation, microbiota, periodontitis, psoriasis, saliva
OBJECTIVE: The purpose of the present study was to characterize the composition of the salivary microbiota and quantify salivary levels of inflammation-related proteins (neutrophil gelatinase-associated lipocalin [NGAL] and transferrin) in patients with psoriasis and compare data to those obtained in patients with periodontitis and orally healthy controls, respectively. MATERIALS AND METHODS: Stimulated saliva samples from patients with psoriasis (n = 27), patients with periodontitis (n = 58), and orally healthy controls (n = 52) were characterized by means of next-generation sequencing of the 16S rRNA gene. Salivary levels of NGAL and transferrin were quantified using immunoassays. RESULTS: Linear discriminant effect size analysis showed that 52 (22 psoriasis-associated and 30 periodontitis-associated) and 21 (8 psoriasis-associated and 13 orally healthy control-associated) bacterial taxa differentiated the salivary microbiota in patients with psoriasis from that of patients with periodontitis and orally healthy controls, respectively. Significantly lower mean salivary levels of NGAL (psoriasis: 996 [std. error 320], periodontitis: 2,072 [295], orally healthy controls: 2,551 [345] ng/ml, p < .0001) and transferrin (psoriasis: 4.37 [0.92], periodontitis: 7.25 [0.88], orally healthy controls: 10.02 [0.94] ng/ml, p < .0001) were identified in patients with psoriasis. CONCLUSIONS: Psoriasis associates with characteristics of the salivary microbiota and salivary levels of inflammation-related proteins, which are different from characteristics in patients with periodontitis and orally healthy controls, respectively.
Experiment 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Curated date: 2023/03/30
Curator: Ellajessica
Revision editor(s): Ellajessica, Aiyshaaaa, Peace Sandy
Subjects
- Location of subjects
- Denmark
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Saliva Sailva normalis,Saliva atomaris,Saliva molecularis,Salivary gland secretion,Saliva,saliva
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Psoriasis Other and unspecified pityriasis,OTHER PSORIASIS,Other psoriasis and similar disorders,Other psoriasis and similar disorders (disorder),Other psoriasis and similar disorders excluding psoriatic arthropathy,Palmoplantaris Pustulosis,PITYRIASIS NEC & NOS,PSORIAS RELATED DIS NEC,Psoriases,psoriasis,Psoriasis and similar disorders,Psoriasis and similar disorders (disorder),Psoriasis and similar disorders (navigational concept),Psoriasis and similar disorders NOS,Psoriasis and similar disorders NOS (disorder),Pustular Psoriasis of Palms and Soles,PUSTULAR PSORIASIS OF PALMS SOLES,Pustulosis of Palms and Soles,PUSTULOSIS OF PALMS SOLES,Pustulosis Palmaris et Plantaris,Psoriasis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- orally healthy individuals
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Psoriasis patient
- Group 0 sample size Number of subjects in the control (unexposed) group
- 52
- Group 1 sample size Number of subjects in the case (exposed) group
- 27
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 3 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- .0001
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(b)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between healthy controls and Psoriasis.
Abundance in Group 1: increased abundance in Psoriasis patient
| NCBI | Quality Control | Links |
|---|---|---|
| Aggregatibacter sp. | ||
| Capnocytophaga sp. | ||
| Haemophilus parainfluenzae | ||
| Neisseria perflava | ||
| Peptoniphilus lacrimalis | ||
| Prevotella melaninogenica | ||
| Rothia mucilaginosa |
Revision editor(s): Ellajessica, Aiyshaaaa
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(b)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between healthy controls and Psoriasis.
Abundance in Group 1: decreased abundance in Psoriasis patient
| NCBI | Quality Control | Links |
|---|---|---|
| Actinomyces sp. | ||
| Arachnia propionica | ||
| Capnocytophaga sp. | ||
| Lactobacillus gasseri | ||
| Neisseria macacae | ||
| Streptococcus mutans | ||
| Streptococcus sobrinus | ||
| Veillonella atypica | ||
| Veillonella sp. |
Revision editor(s): Ellajessica, Aiyshaaaa
Experiment 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- periodntitis
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Psoriasis
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Individuals who have been diagnosed with Psoriasis.
- Group 0 sample size Number of subjects in the control (unexposed) group
- 27
- Group 1 sample size Number of subjects in the case (exposed) group
- 58
Lab analysis
Statistical Analysis
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- .001
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(a)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between Periodontitis (P) versus Psoriasis (PS).
Abundance in Group 1: increased abundance in Psoriasis
Revision editor(s): Aiyshaaaa
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(a)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between Periodontitis (P) versus Psoriasis (PS).
Abundance in Group 1: decreased abundance in Psoriasis
Revision editor(s): Aiyshaaaa
Experiment 3
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Periodontitis chronic pericementitis,inflammation of periodontium,Pericementitides,Pericementitis,Periodontitides,periodontitis,Periodontitis (disorder),Periodontitis, NOS,periodontium inflammation,periodontosis,Periodontitis
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- orally healthy individuals
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Periodontitis patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Patients who have been diagnosed with periodontitis
- Group 0 sample size Number of subjects in the control (unexposed) group
- 52
- Group 1 sample size Number of subjects in the case (exposed) group
- 27
Lab analysis
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- Not specified
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- unchanged
- Chao1 Abundance-based estimator of species richness
- unchanged
- Richness Number of species
- unchanged
Signature 1
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(c)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between healthy controls versus Periodontitis (P).
Abundance in Group 1: increased abundance in Periodontitis patients
Revision editor(s): Atrayees
Signature 2
Reviewed Marked as Reviewed by Atrayees on 2023-6-1
Source: Figure 2(c)
Description: Using Linear discriminant effect size analysis. Linear discriminant analysis score expressed as (log 10) of significant bacterial taxa between healthy controls versus Periodontitis (P).
Abundance in Group 1: decreased abundance in Periodontitis patients
Revision editor(s): Atrayees
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