The Gut Microbiome Is Altered in a Letrozole-Induced Mouse Model of Polycystic Ovary Syndrome

From BugSigDB
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Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Authors
Kelley ST, Skarra DV, Rivera AJ, Thackray VG
Journal
PloS one
Year
2016
Women with polycystic ovary syndrome (PCOS) have reproductive and metabolic abnormalities that result in an increased risk of infertility, diabetes and cardiovascular disease. The large intestine contains a complex community of microorganisms (the gut microbiome) that is dysregulated in humans with obesity and type 2 diabetes. Using a letrozole-induced PCOS mouse model, we demonstrated significant diet-independent changes in the gut microbial community, suggesting that gut microbiome dysbiosis may also occur in PCOS women. Letrozole treatment was associated with a time-dependent shift in the gut microbiome and a substantial reduction in overall species and phylogenetic richness. Letrozole treatment also correlated with significant changes in the abundance of specific Bacteroidetes and Firmicutes previously implicated in other mouse models of metabolic disease in a time-dependent manner. Our results suggest that the hyperandrogenemia observed in PCOS may significantly alter the gut microbiome independently of diet.

Experiment 1


incomplete

Curated date: 2022/12/05

Curator: Testuser

Revision editor(s): Testuser

Subjects

Location of subjects
United States of America
Host species Species from which microbiome was sampled (if applicable)
Mus musculus
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Presumptive gut Embryonic digestive tube,Future digestive tract,Future digestive tube,Future gut,Primitive gut,Primordial digestive tube,Primordial gut,Presumptive gut
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Placebo-treated
Group 1 name Corresponds to the case (exposed) group for case-control studies
Letrozole-treated
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
At 4 weeks of age, the mice were implanted subcutaneously with a 3 mg letrozole pellet (Innovative Research of America, Sarasota, FL) that provided a constant, slow release of letrozole (50 μg/day) (n = 10/group).

Control (Group 0 mice were implanted subcutaneously with a placebo pellet

Group 0 sample size Number of subjects in the control (unexposed) group
10
Group 1 sample size Number of subjects in the case (exposed) group
10
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
N/A

Lab analysis

Sequencing type
16S
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Statistical test
Kruskall-Wallis
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
Yes


Alpha Diversity

Chao1 Abundance-based estimator of species richness
decreased

Signature 1

incomplete

Curated date: 2022/12/05

Curator: Testuser

Revision editor(s): Testuser

Source:

Description:

Abundance in Group 1: decreased abundance in Letrozole-treated

NCBI Links
Muribaculaceae
Ruminococcus
Alistipes

Revision editor(s): Testuser

Signature 2

incomplete

Curated date: 2022/12/05

Curator: Testuser

Revision editor(s): Testuser

Source: S2 Fig

Description: Letrozole treatment results in significant changes in 48 bacterial OTUs as assessed by Kruskal-Wallis test.

The denovo OTU identifier, Test-Statistic, uncorrected p-value, corrected p-value using FDR and Bonferroni correction for multiple comparisons, placebo mean of ranked relative abundance over the entire study (weeks 0–5), letrozole mean from weeks 0–5, and the taxonomy of the closest match as identified using Greengenes are shown.

https://doi.org/10.1371/journal.pone.0146509.s002

Abundance in Group 1: increased abundance in Letrozole-treated

NCBI Links
Allobaculum
Blautia

Revision editor(s): Testuser