The Gut Microbiome Is Altered in a Letrozole-Induced Mouse Model of Polycystic Ovary Syndrome
- Location of subjects
- United States of America
- Host species Species from which microbiome was sampled (if applicable)
- Mus musculus
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- polycystic ovary syndrome Cystic disease of ovaries,hyperandrogenemia,Multicystic ovaries,multicystic ovaries,Ovarian Degeneration, Sclerocystic,Ovarian Syndrome, Polycystic,Ovarian Syndromes, Polycystic,Ovaries, Sclerocystic,Ovary Syndrome, Polycystic,Ovary, Sclerocystic,PCO - Polycystic ovaries,Pco1,PCOD - Polycystic ovarian disease,PCOS,Pcos,PCOS - Polycystic ovarian syndrome,PCOS1,Polycystic ovarian disease,polycystic ovarian disease,Polycystic ovarian syndrome,Polycystic ovaries,polycystic ovaries,Polycystic ovaries (disorder),polycystic ovary,polycystic ovary syndrome,polycystic ovary syndrome 1,Sclerocystic Ovarian Degeneration,Sclerocystic Ovaries,Sclerocystic Ovary,Sclerocystic Ovary Syndrome,Stein Leventhal Syndrome,Stein-Leventhal synd.,Stein-Leventhal Syndrome,Stein-Leventhal syndrome,Syndrome, Polycystic Ovary,Syndrome, Stein-Leventhal
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- At 4 weeks of age, the mice were implanted subcutaneously with a 3 mg letrozole pellet (Innovative Research of America, Sarasota, FL) that provided a constant, slow release of letrozole (50 μg/day) (n = 10/group).
- Group 0 sample size Number of subjects in the control (unexposed) group
- Group 1 sample size Number of subjects in the case (exposed) group
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- Sequencing type
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Statistical test
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Chao1 Abundance-based estimator of species richness
Source: S2 Fig
Description: Letrozole treatment results in significant changes in 48 bacterial OTUs as assessed by Kruskal-Wallis test.
The denovo OTU identifier, Test-Statistic, uncorrected p-value, corrected p-value using FDR and Bonferroni correction for multiple comparisons, placebo mean of ranked relative abundance over the entire study (weeks 0–5), letrozole mean from weeks 0–5, and the taxonomy of the closest match as identified using Greengenes are shown.
Abundance in Group 1: increased abundance in Letrozole-treated
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