Gut Microbiota Altered in Mild Cognitive Impairment Compared With Normal Cognition in Sporadic Parkinson's Disease

Study information

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

32161568

DOI Digital object identifier for electronic documents.

10.3389/fneur.2020.00137

URI

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052381/

Authors

Ren T, Gao Y, Qiu Y, Jiang S, Zhang Q, Zhang J, Wang L, Zhang Y, Wang L, Nie K

Journal

Frontiers in neurology

Year

2020

Keywords:

Parkinson's disease, cognition impairment, gut micro biome, high throughput sequencing, short fatty acids

Background and Aim: Gut bacteria play an important role in the pathogenesis of Parkinson's disease (PD). However, the alteration of fecal microbiota in PD with cognitive impairment remains unexplored. This study aimed to explore whether the gut microbiota of patients with PD having mild cognitive impairment (PD-MCI) were different from those with PD having normal cognition (PD-NC) and from healthy controls (HC). Also, the study probed the association between altered gut microbiota and cognitive ability in patients with PD. Methods: The fecal bacteria composition and short-chain fatty acids of 13 patients with PD-MCI, 14 patients with PD-NC, and 13 healthy spouses were analyzed using 16S ribosomal RNA sequencing and gas chromatography-mass spectrometry. Results: Compared with HC, the fecal microbial diversities increased in patients with PD-MCI and PD-NC. After adjusting the influence of age, sex, body mass index, education, and constipation using the statistical method, the relative abundances of two families (Rikenellaceae and Ruminococcaceae) and four genera (Alistipes, Barnesiella, Butyricimonas, and Odoribacter) were found to be higher in the feces of the PD-MCI group compared with the other two groups. Moreover, the abundance of genus Blautia and Ruminococcus decreased obviously in the PD-MCI group compared with the PD-NC group. Further, the abundance of genera Butyricimonas, Barnesiella, Alistipes, Odoribacter, and Ruminococcus negatively correlated with cognition ability. Conclusion: Compared with HC and patients with PD-NC, the gut microbiota of patients with PD-MCI was significantly altered, particularly manifesting in enriched genera from Porphyromonadaceae family and decreased the abundance of genera Blautia and Ruminococcus.

Experiment 1

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Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Subjects

Location of subjects: China

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Parkinson's disease IDIOPATHIC PARKINSON DIS,Idiopathic Parkinson Disease,Idiopathic Parkinson's Disease,IDIOPATHIC PARKINSONS DIS,Idiopathic PD,LEWY BODY PARKINSON DIS,Lewy Body Parkinson Disease,Lewy Body Parkinson's Disease,Paralysis agitans,paralysis agitans,PARKINSON DIS,PARKINSON DIS IDIOPATHIC,Parkinson disease,Parkinson Disease, Idiopathic,Parkinson syndrome,Parkinson's,Parkinson's disease,Parkinson's disease (disorder),Parkinson's disease NOS,Parkinson's disease NOS (disorder),Parkinson's Disease, Idiopathic,Parkinson's Disease, Lewy Body,Parkinson's syndrome,Parkinsonian disorder,Parkinsonism, Primary,Parkinsons,PARKINSONS DIS,PARKINSONS DIS IDIOPATHIC,PARKINSONS DIS LEWY BODY,Parkinsons disease,Primary Parkinsonism,parkinson's disease

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy spouses and Parkinson's disease patients with mild cognitive impairment

Group 1 name Corresponds to the case (exposed) group for case-control studies: Parkinson's disease patients with normal cognition

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: All patients eligible for this study were diagnosed for PD according to the UK Brain Bank criteria. Cognition abilities were estimated using the Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA), and the scores were obtained from two other neuropsychological tests in each of the five cognitive domains.

Group 0 sample size Number of subjects in the control (unexposed) group: 26

Group 1 sample size Number of subjects in the case (exposed) group: 14

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): .05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2.0

Matched on Factors on which subjects have been matched on in a case-control study: age

Confounders controlled for Confounding factors that have been accounted for by stratification or model adjustment: age, body mass index, education level, sex, constipation

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

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Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Source: Figure S3

Description: Alteration in the taxa between PD-MCI, PD-NC and healthy groups(LDA)

Abundance in Group 1: increased abundance in Parkinson's disease patients with normal cognition

NCBI	Quality Control	Links
Eubacteriales
Clostridia
Oscillospiraceae
Porphyromonadaceae
uncultured Oscillospiraceae bacterium
Ruminococcus
Blautia
Actinomycetota
Christensenellaceae
Parabacteroides goldsteinii
Ruminococcus callidus
Blautia producta
Coriobacteriaceae
Coriobacteriia
Coriobacteriales

Revision editor(s): Jacquelynshevin

Experiment 2

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Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy spouses and Parkinson's disease patients with normal cognition

Group 1 name Corresponds to the case (exposed) group for case-control studies: Parkinson's disease patients with mild cognitive impairment

Group 0 sample size Number of subjects in the control (unexposed) group: 27

Group 1 sample size Number of subjects in the case (exposed) group: 13

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: unchanged

Simpson Estimator of species richness and species evenness: more weight on species evenness: unchanged

Signature 1

Edit History Delete

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Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Source: Figure S3

Description: Alteration in the taxa between PD-MCI, PD-NC and healthy groups(LDA)

Abundance in Group 1: increased abundance in Parkinson's disease patients with mild cognitive impairment

NCBI	Quality Control	Links
Rikenellaceae
Alistipes
Ruminococcus sp. N15.MGS-57
Klebsiella
Klebsiella pneumoniae
Barnesiella
Butyricimonas
Anaerotruncus
[Ruminococcus] torques
Odoribacter
Odoribacter splanchnicus

Revision editor(s): Jacquelynshevin

Experiment 3

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Your review change was submittedDuplicate this Experiment Add a new Signature

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy spouses

Group 1 name Corresponds to the case (exposed) group for case-control studies: Parkinson's disease patients with mild cognitive impairment and Parkinson's disease patients with normal cognition

Group 0 sample size Number of subjects in the control (unexposed) group: 13

Group 1 sample size Number of subjects in the case (exposed) group: 27

Lab analysis

Statistical Analysis

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Chao1 Abundance-based estimator of species richness: increased

Simpson Estimator of species richness and species evenness: more weight on species evenness: increased

Signature 1

Edit History Delete

Flag for review

Your review change was submitted

Reviewed Marked as Reviewed by Atrayees on 2023-7-3

Curated date: 2023/05/20

Curator: Jacquelynshevin

Revision editor(s): Jacquelynshevin

Source: Figure S3

Description: Alteration in the taxa between PD-MCI, PD-NC and healthy groups(LDA)

Abundance in Group 1: decreased abundance in Parkinson's disease patients with mild cognitive impairment and Parkinson's disease patients with normal cognition

NCBI	Quality Control	Links
Bacteroidales
Bacteroidia
Megasphaera micronuciformis
Veillonella
Bacteroidota

Revision editor(s): Jacquelynshevin