Whole metagenome profiling reveals skin microbiome-dependent susceptibility to atopic dermatitis flare/Experiment 3

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Reviewed Marked as Reviewed by Lwaldron on 2023-4-1

Curated date: 2023/03/26

Curator: Ufuoma Ejite

Revision editor(s): Ufuoma Ejite, Lwaldron

Subjects

Location of subjects

Singapore

Host species Species from which microbiome was sampled. Contact us to have more species added.

Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology

Skin epithelium Skin epithelium,skin epithelium

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology

Eczema Eczema,eczema

Group 0 name Corresponds to the control (unexposed) group for case-control studies

Males (control)

Group 1 name Corresponds to the case (exposed) group for case-control studies

Males (case)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group

Atopic dermatitis (AD) case males

Group 0 sample size Number of subjects in the control (unexposed) group

8

Group 1 sample size Number of subjects in the case (exposed) group

8

Lab analysis

Sequencing type

WMS

16S variable region One or more hypervariable region(s) of the bacterial 16S gene

Not specified

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance

Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).

relative abundances

Statistical test

Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any)

0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Yes

Signature 1

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Reviewed Marked as Reviewed by Lwaldron on 2023-4-2

Curated date: 2023/03/30

Curator: Ufuoma Ejite

Revision editor(s): Ufuoma Ejite, Lwaldron, WikiWorks

Source: Supplementary Figure 6B

Description: Differential microbial abundance between male controls and male AD case group

Abundance in Group 1: decreased abundance in Males (case)

NCBI	Quality Control	Links
Deinococcus
Dermacoccus

Revision editor(s): Ufuoma Ejite, Lwaldron, WikiWorks

Signature 2

EditHistoryDelete

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Reviewed Marked as Reviewed by Lwaldron on 2023-4-2

Curated date: 2023/03/30

Curator: Ufuoma Ejite

Revision editor(s): Ufuoma Ejite, Lwaldron, WikiWorks

Source: Supplementary Figure 6B

Description: Differential microbial abundance between male controls and male AD case group

Abundance in Group 1: increased abundance in Males (case)

NCBI	Quality Control	Links
Streptococcus
Gemella
Veillonella
Haemophilus

Revision editor(s): Ufuoma Ejite, Lwaldron, WikiWorks