Role of a probiotic strain in the modulation of gut microbiota and cytokines in inflammatory bowel disease

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PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI Uniform resource identifier for web resources.
Bamola VD, Dubey D, Samanta P, Kedia S, Ahuja V, Madempudi RS, Neelamraju J, Chaudhry R
OBJECTIVE: To assess the effect of a probiotic strain Bacillus clausii UBBC-07 on gut microbiota and cytokines in IBD patients. METHOD: Patients were randomly allocated to either placebo or probiotic Bacillus clausii UBBC-07 for four weeks along with the standard medical treatment (SMT). Enrolled patients were evaluated before and after intervention for presence of the given probiotic, change in gut microbiota, change in serum cytokines, serotonin and dopamine, symptoms of disease, physical, behavioral and psychological parameters. RESULTS: Probiotic strain Bacillus clausii UBBC-07 showed good survival in IBD patients in the treatment group (p < 0.01) without any reported adverse event. Metagenomic analysis showed that the given probiotic strain was able to modulate the gut microbiota in treated group. Phylum Firmicutes was increased and phylum Bacteroidetes was decreased in the probiotic treated group. A significant increase was observed in the abundance of anaerobic bacterial genera Lactobacillus, Bifidobacterium and Faecalibacterium in the probiotic treated group (p < 0.01) as compared to placebo group. Significant increase was observed in IL-10 (p < 0.05) and variable decrease in the secretion of IL-1β, TNF- α, IL-6, IL -17 and IL -23 in probiotic treated group. In the treatment group a significant decrease in the symptoms of IBD and improvement in the psychological parameter to various degrees was noted. CONCLUSION: These results indicated that probiotic strain B clausii UBBC-07 affected the gut microbiota and cytokine secretion and shown efficacy in IBD patients.

Experiment 1

Needs review

Curated date: 2023/03/11

Curator: Brian

Revision editor(s): Brian


Location of subjects
Host species Species from which microbiome was sampled (if applicable)
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Anterior ectodermal midgut Anterior ectodermal midgut
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
response to placebo , gestational serum measurement , gut microbiome measurement , response to serotonin , dopamine response to placebo,gestational serum measurement,gut microbiome measurement,response to serotonin,2-(3,4-Dihydroxyphenyl)ethylamine,2-(3,4-dihydroxyphenyl)ethylamine,3,4-Dihydroxyphenethylamine,3-Hydroxytyramine,4-(2-Aminoethyl)-1,2-benzenediol,4-(2-aminoethyl)-1,2-benzenediol,4-(2-Aminoethyl)benzene-1,2-diol,4-(2-aminoethyl)benzene-1,2-diol,4-(2-aminoethyl)catechol,4-(2-aminoethyl)pyrocatechol,C8H11NO2,Deoxyepinephrine,dopamina,Dopamine,dopamine,dopaminum,Hydroxytyramin
Group 0 name Corresponds to the control (unexposed) group for case-control studies
healthy conrols
Group 1 name Corresponds to the case (exposed) group for case-control studies
response to placebo
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients were randomly allocated to either placebo or probiotic Bacillus clausii UBBC-07 for four weeks along with the standard medical treatment (SMT).
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
4 weeks

Lab analysis

Sequencing type
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Mass spectrometry

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?

Alpha Diversity

Pielou Quantifies how equal the community is numerically
Shannon Estimator of species richness and species evenness: more weight on species richness
Chao1 Abundance-based estimator of species richness
Simpson Estimator of species richness and species evenness: more weight on species evenness
Richness Number of species