Human intestinal microbiota composition is associated with local and systemic inflammation in obesity
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Verdam FJ, Fuentes S, de Jonge C, Zoetendal EG, Erbil R, Greve JW, Buurman WA, de Vos WM, Rensen SS
Journal
Obesity (Silver Spring, Md.)
Year
2013
OBJECTIVE: Intestinal microbiota have been suggested to contribute to the development of obesity, but the mechanism remains elusive. The relationship between microbiota composition, intestinal permeability, and inflammation in nonobese and obese subjects was investigated. DESIGN AND METHODS: Fecal microbiota composition of 28 subjects (BMI 18.6-60.3 kg m(-2) ) was analyzed by a phylogenetic profiling microarray. Fecal calprotectin and plasma C-reactive protein levels were determined to evaluate intestinal and systemic inflammation. Furthermore, HbA1c , and plasma levels of transaminases and lipids were analyzed. Gastroduodenal, small intestinal, and colonic permeability were assessed by a multisaccharide test. RESULTS: Based on microbiota composition, the study population segregated into two clusters with predominantly obese (15/19) or exclusively nonobese (9/9) subjects. Whereas intestinal permeability did not differ between clusters, the obese cluster showed reduced bacterial diversity, a decreased Bacteroidetes/Firmicutes ratio, and an increased abundance of potential proinflammatory Proteobacteria. Interestingly, fecal calprotectin was only detectable in subjects within the obese microbiota cluster (n = 8/19, P = 0.02). Plasma C-reactive protein was also increased in these subjects (P = 0.0005), and correlated with the Bacteroidetes/Firmicutes ratio (rs = -0.41, P = 0.03). CONCLUSIONS: Intestinal microbiota alterations in obese subjects are associated with local and systemic inflammation, suggesting that the obesity-related microbiota composition has a proinflammatory effect.
Experiment 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Subjects
- Location of subjects
- Netherlands
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Obesity Adiposis,Adiposity,Obese,Obese (finding),obesity,Obesity (disorder),Obesity [Ambiguous],obesity disease,obesity disorder,Obesity NOS,Obesity, unspecified,Overweight and obesity,Obesity
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- non-obese
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- obese
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- obesity: BMI 30.5-60.3 kg/m2
- Group 0 sample size Number of subjects in the control (unexposed) group
- 13
- Group 1 sample size Number of subjects in the case (exposed) group
- 15
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- 6 months
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- Not specified
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Human Intestinal Tract Chip
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- Mann-Whitney (Wilcoxon)
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- Yes
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2
Description: Differential microbial abundance between obese and non-obese individuals
Abundance in Group 1: increased abundance in obese
| NCBI | Quality Control | Links |
|---|---|---|
| Dorea formicigenerans | ||
| Papillibacter cinnamivorans | ||
| [Clostridium] symbiosum |
Revision editor(s): WikiWorks, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2
Description: Differential microbial abundance between obese and non-obese individuals
Abundance in Group 1: decreased abundance in obese
Revision editor(s): WikiWorks, ChiomaBlessing
Experiment 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Nonobese microbiota cluster
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- obesity: BMI 30.5-60.3 kg/m3
Lab analysis
Statistical Analysis
Alpha Diversity
- Inverse Simpson Modification of Simpsons index D as 1/D to obtain high values in datasets of high diversity and vice versa
- decreased
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2
Description: Association of intestinal microbiota with local and systemic inflammation in obesity
Abundance in Group 1: increased abundance in obese
| NCBI | Quality Control | Links |
|---|---|---|
| [Clostridium] symbiosum | ||
| [Clostridium] colinum | ||
| Anaerobutyricum hallii |
Revision editor(s): WikiWorks
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 2
Description: Association of intestinal microbiota with local and systemic inflammation in obesity
Abundance in Group 1: decreased abundance in obese
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Bacteroides fragilis | ||
| Bacteroides intestinalis | ||
| Odoribacter splanchnicus | ||
| Oscillospira guilliermondii |
Revision editor(s): WikiWorks
Experiment 3
Reviewed Marked as Reviewed by Fatima on 2021/07/16
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low BMI
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high BMI
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- obesity: BMI 30.5-60.3 kg/m4
Lab analysis
Statistical Analysis
- Statistical test
- Spearman Correlation
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Differential microbial abundance between high-BMI and low-BMI individuals
Abundance in Group 1: increased abundance in high BMI
Revision editor(s): WikiWorks, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Differential microbial abundance between high-BMI and low-BMI individuals
Abundance in Group 1: decreased abundance in high BMI
Revision editor(s): WikiWorks, ChiomaBlessing
Experiment 4
Reviewed Marked as Reviewed by Fatima on 2021/07/16
Differences from previous experiment shown
Subjects
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- low C-Reactive Protein
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- high C-Reactive Protein
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- obesity: BMI 30.5-60.3 kg/m5
Lab analysis
Statistical Analysis
Signature 1
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Differential microbial abundance between high C-reactive protein group and low C-reactive protein group
Abundance in Group 1: increased abundance in high C-Reactive Protein
| NCBI | Quality Control | Links |
|---|---|---|
| Aneurinibacillus | ||
| Lacrimispora sphenoides | ||
| Papillibacter cinnamivorans | ||
| Roseburia intestinalis | ||
| Subdoligranulum variabile |
Revision editor(s): WikiWorks, ChiomaBlessing
Signature 2
Reviewed Marked as Reviewed by Shaimaa Elsafoury on 2021/02/09
Source: Table 3
Description: Differential microbial abundance between high C-reactive protein group and low C-reactive protein group
Abundance in Group 1: decreased abundance in high C-Reactive Protein
| NCBI | Quality Control | Links |
|---|---|---|
| Alistipes | ||
| Bacteroides intestinalis | ||
| Odoribacter splanchnicus | ||
| Parabacteroides distasonis | ||
| Phocaeicola plebeius | ||
| Phocaeicola vulgatus | ||
| Tannerella |
Revision editor(s): WikiWorks, ChiomaBlessing
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