Postoperative Probiotics Administration Attenuates Gastrointestinal Complications and Gut Microbiota Dysbiosis Caused by Chemotherapy in Colorectal Cancer Patients

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
URI
Authors
Huang F, Li S, Chen W, Han Y, Yao Y, Yang L, Li Q, Xiao Q, Wei J, Liu Z, Chen T, Deng X
Journal
Nutrients
Year
2023
Keywords:
CRC, chemotherapy, gastrointestinal complications, gut microbiota, probiotics
The current study aims to evaluate the potential roles of taking probiotics postoperatively in attenuating the gastrointestinal complications and disturbed gut microbiota in colorectal cancer (CRC) patients undergoing chemotherapy. One hundred eligible CRC patients who were treated with radical surgery and needed to receive chemotherapy were recruited. Half of them were randomly assigned to the Probio group to take a probiotic combination from post-operation to the end of the first chemotherapeutic course. The other half of patients taking placebo instead were classified as the Placebo group. Gastrointestinal complications such as nausea, acid reflux, abdominal pain, abdominal distention, constipation, and diarrhea were recorded during chemotherapy. Fecal samples were collected preoperatively and after the first cycle of postoperative chemotherapy for 16S rRNA high-throughput sequencing and short-chain fatty acids (SCFAs) analysis. Results showed that probiotics administration could effectively reduce chemotherapy-induced gastrointestinal complications, particularly in diarrhea (p < 0.01). Additionally, chemotherapy also reduced the bacterial diversity indexes of the gut microbiota in CRC patients, which could be significantly increased by taking probiotics. Moreover, this chemotherapy caused significant changes in the composition of the gut microbiota, as indicated by decreased phylum levels of Firmicutes and increased Bacteroidetes, Proteobacteria, and Verrucomicrobia. In particular, several bacterial genera such as Akkermansia and Clostridium were significantly increased, while Prevotella, Lactobacillus, and Roseburia were decreased (p < 0.05). However, probiotic administration could effectively restore these taxa changes both at the phylum and genus levels, and mildly increase the genus levels of Bifidobacterium, Streptococcus, and Blautia. Furthermore, probiotics could also promote the production of SCFAs, particularly increasing acetate, butyrate, and propionate (p < 0.0001). These results support the beneficial effects of the probiotic interventions as novel alternative or complementary strategies in chemoprevention.

Experiment 1


Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2023/09/07

Curator: Yjung24

Revision editor(s): Yjung24, Peace Sandy

Subjects

Location of subjects
China
Host species Species from which microbiome was sampled. Contact us to have more species added.
Homo sapiens
Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
Feces Cow dung,Cow pat,Droppings,Dung,Excrement,Excreta,Faeces,Fecal material,Fecal matter,Fewmet,Frass,Guano,Matières fécales@fr,Merde@fr,Ordure,Partie de la merde@fr,Piece of shit,Porción de mierda@es,Portion of dung,Portion of excrement,Portion of faeces,Portion of fecal material,Portion of fecal matter,Portion of feces,Portion of guano,Portion of scat,Portionem cacas,Scat,Spoor,Spraint,Stool,Teil der fäkalien@de,Feces,feces
Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
Colorectal cancer cancer of colorectum,cancer of large bowel,cancer of large intestine,cancer of the large bowel,colon cancer,colorectal cancer,colorectum cancer,CRC,large intestine cancer,malignant colorectal neoplasm,malignant colorectal tumor,malignant colorectum neoplasm,malignant large bowel neoplasm,malignant large bowel tumor,malignant large intestine neoplasm,malignant large intestine tumor,malignant neoplasm of colorectum,malignant neoplasm of large bowel,malignant neoplasm of large intestine,malignant neoplasm of the large bowel,malignant neoplasm of the large intestine,malignant tumor of large bowel,malignant tumor of large intestine,malignant tumor of the large bowel,malignant tumor of the large intestine,Colorectal cancer
Group 0 name Corresponds to the control (unexposed) group for case-control studies
Placebo control group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Probio group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients in the Probio group were instructed to take probiotic tablets orally
Group 0 sample size Number of subjects in the control (unexposed) group
50
Group 1 sample size Number of subjects in the case (exposed) group
50
Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
2 Weeks

Lab analysis

Sequencing type
16S
16S variable region One or more hypervariable region(s) of the bacterial 16S gene
V4
Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
relative abundances
Statistical test
LEfSe
Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
0.05
MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
No
LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
2

Alpha Diversity

Pielou Quantifies how equal the community is numerically
increased
Shannon Estimator of species richness and species evenness: more weight on species richness
increased
Chao1 Abundance-based estimator of species richness
increased

Signature 1

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2023/09/14

Curator: Yjung24

Revision editor(s): Yjung24, Atrayees, Peace Sandy

Source: Figure 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: increased abundance in Probio group

NCBI Quality ControlLinks
Rubrobacteria
Rubrobacterales
Micrococcaceae
Rubrobacteraceae
Rikenellaceae
Turicibacteraceae
Clostridiaceae
Peptococcaceae
Rothia
Rubrobacter
Odoribacter
Alistipes
Turicibacter
Clostridium
Blautia
Lactonifactor
Peptococcus
Anaerotruncus
Haemophilus

Revision editor(s): Yjung24, Atrayees, Peace Sandy

Signature 2

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2023/09/14

Curator: Yjung24

Revision editor(s): Yjung24, Peace Sandy

Source: Figure 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: decreased abundance in Probio group

NCBI Quality ControlLinks
Abiotrophia
Actinobacillus
Aerococcaceae
Akkermansia
Butyricicoccus
Christensenella sp.
Enterococcaceae
Erysipelotrichaceae
Jeotgalicoccus
Lachnospira
Lachnospiraceae
Morganella
Parabacteroides
Paraeggerthella
Paraprevotella
Pseudomonadaceae
Psychrobacter
Ruminococcus
Verrucomicrobiaceae
Verrucomicrobiae
Verrucomicrobiales
Verrucomicrobiota

Revision editor(s): Yjung24, Peace Sandy

Experiment 2


Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2024/02/01

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
CRC [colorectal cancer] Patients Pre-treatment group

Lab analysis

Statistical Analysis

Alpha Diversity

Pielou Quantifies how equal the community is numerically
unchanged
Shannon Estimator of species richness and species evenness: more weight on species richness
unchanged
Chao1 Abundance-based estimator of species richness
unchanged

Signature 1

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2024/02/01

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Source: Fig 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: increased abundance in Probio group

NCBI Quality ControlLinks
Alistipes
Anaerorhabdus
Anaerotruncus
Blautia
Clostridiaceae
Clostridiaceae bacterium
Gemmiger
Haemophilus
Lactonifactor
Micrococcaceae
Odoribacter
Peptococcaceae
Peptococcus
Rikenellaceae
Rothia
Rubrobacter
Rubrobacteraceae
Rubrobacterales
Rubrobacteria
Turicibacter
Turicibacteraceae

Revision editor(s): Peace Sandy

Signature 2

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2024/02/01

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Source: Fig 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: decreased abundance in Probio group

NCBI Quality ControlLinks
Lentisphaerota
Gemmatimonadetes
Opitutaceae bacterium TAV5
Clostridia
Lentisphaeria
Eubacteriales
Victivallales
Rhodocyclales
Comamonadaceae
Rhodocyclaceae
Lactobacillaceae
Victivallaceae
Adlercreutzia
Lactobacillus
Gallicola
Anaerostipes
Butyrivibrio
Coprococcus
Roseburia
Phascolarctobacterium
Victivallis
Desulfovibrio

Revision editor(s): Peace Sandy

Experiment 3


Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2024/02/01

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies
CRC [colorectal cancer] Patients Pre-treatment group
Group 1 name Corresponds to the case (exposed) group for case-control studies
Placebo group
Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
Patients in the Placebo group routinely treated with placebo tablets instead of probiotics

Lab analysis

Statistical Analysis

Alpha Diversity

Pielou Quantifies how equal the community is numerically
decreased
Shannon Estimator of species richness and species evenness: more weight on species richness
decreased
Chao1 Abundance-based estimator of species richness
decreased

Signature 1

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2023/09/14

Curator: Yjung24

Revision editor(s): Yjung24, Peace Sandy

Source: Figure 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: increased abundance in Placebo group

NCBI Quality ControlLinks
Abiotrophia
Actinobacillus
Aerococcaceae
Akkermansia
Butyricicoccus
Christensenella sp.
Enterococcaceae
Erysipelotrichaceae
Jeotgalicoccus
Lachnospira
Lachnospiraceae
Morganella
Parabacteroides
Paraeggerthella
Pseudomonadaceae
Psychrobacter
Verrucomicrobiaceae
Verrucomicrobiae
Verrucomicrobiales
Verrucomicrobiota

Revision editor(s): Yjung24, Peace Sandy

Signature 2

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-2

Curated date: 2024/02/02

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Source: Fig 4

Description: LEfSe cladogram showing differently abundant gut microbiota taxa among CRC patients at different levels. The current LDA threshold score is over 2; p, phylum; c, class; o, order; f, family; g, genus. The blue, red, and green color refers to different bacterial taxa in CRC group, Placebo, and Probio group, respectively.

Abundance in Group 1: decreased abundance in Placebo group

NCBI Quality ControlLinks
Adlercreutzia
Anaerostipes
Butyrivibrio
Clostridia
Comamonadaceae
Coprococcus
Desulfovibrio
Eubacteriales
Gallicola
Gemmatimonadetes
Lactobacillaceae
Lactobacillus
Lentisphaeria
Lentisphaerota
Opitutae
Phascolarctobacterium
Rhodocyclaceae
Rhodocyclales
Victivallaceae
Victivallales
Victivallis

Revision editor(s): Peace Sandy