Vaginal microbiome of women with adenomyosis: A case-control study

Study information

Reviewed Marked as Reviewed by Folakunmi on 2024-1-8

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

35171931

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0263283

URI

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0263283

Authors

Kunaseth J, Waiyaput W, Chanchaem P, Sawaswong V, Permpech R, Payungporn S, Sophonsritsuk A

Journal

PloS one

Year

2022

Immune dysregulation can involve invasion and survival of endometrial glands inside the myometrium of the adenomyosis. There is limited available data concerning alterations of the bacterial microbiome in the reproductive tract of adenomyosis women. The present cross-sectional age-matched study aims to compare vaginal microbiota between women with and without adenomyosis. We recruited women with adenomyosis (N = 40) and age-matched women without adenomyosis (N = 40) from the Departments of Obstetrics and Gynaecology, Ramathibodi Hospital Mahidol University, from August 2020 to January 2021. Vaginal swab samples were collected from the participants. DNA isolation and bacterial 16s rDNA gene sequencing and data analyses were then performed. Comparison of the diversity of vaginal microbiota, microbiota composition, and the operational taxonomic unit (OTU) between adenomyosis and non-adenomyosis (control) groups were undertaken. Data from 40 and 38 women with and without adenomyosis, respectively, were analyzed. Alpha-diversity analysis (Chao1 index) at the species level showed higher vaginal microbial richness in the adenomyosis group when compared with the control group (p = 0.006). The linear discriminant analysis effect size technique (LeFSe) indicated an elevated abundance of several vaginal microbial taxa in the adenomyosis group, including Alloscardovia, Oscillospirales, Ruminoccoccaceae, UCG_002, Oscillospiraceae, Enhydrobacter, Megamonas, Moraxellaceae, Subdoligranulum, Selenomonadaceae, and Faecalibacterium. On the other hand, an increase in the abundance of Megaspehera, Fastidiosipila, Hungateiclostridiaceae, and Clostridia was identified in the control group. Vaginal community state type (CST)-III and -IV were dominated in adenomyosis, while only CST-IV was dominated in the non-adenomyosis group. Lactobacillus was the most abundant vaginal microbial in both groups. In this study, the differences in vaginal microbiome profile were noted between adenomyosis and non-adenomyosis group. The increasing of microbial richness was associated with adenomyosis. Nevertheless, further investigations were required to elucidate the mechanisms and apply them for clinical implications.

Experiment 1

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-8

Curated date: 2023/09/24

Curator: Andre

Revision editor(s): Andre, Peace Sandy, Folakunmi

Subjects

Location of subjects: Thailand

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Upper part of vagina Cranial vagina,Pelvic part of vagina,Upper third of vagina,Upper vagina,Vagina upper part,Upper part of vagina,upper part of vagina

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Adenomyosis uterine corpus adenomyosis,Adenomyosis,adenomyosis

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: adenomyosis patients

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Patients with adenomoysis diagnosed using at least three diagnostic ultrasound-based criteria: globular shape uterus, asymmetric myometrial wall, thickening of the endometrial myometrial junction, hyperechoic striae along the sub-endometrial region, and presence of sub-endometrial microcyst

Group 0 sample size Number of subjects in the control (unexposed) group: 38

Group 1 sample size Number of subjects in the case (exposed) group: 40

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Not mentioned

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: LEfSe

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool: 2

Matched on Factors on which subjects have been matched on in a case-control study: age

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: unchanged

Chao1 Abundance-based estimator of species richness: increased

Signature 1

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Curated date: 2023/09/24

Curator: Andre

Revision editor(s): Andre, Peace Sandy, Folakunmi

Source: Fig 4

Description: Linear discriminant analysis effect size (LEfSe) analysis of microbial abundance in the vaginal sample between adenomyosis and normal uterus.

Abundance in Group 1: increased abundance in adenomyosis patients

NCBI	Quality Control	Links
Alloscardovia
Coriobacteriaceae
Enhydrobacter
Faecalibacterium
Megamonas
Moraxellaceae
Oscillospira
Oscillospiraceae
Selenomonadaceae
Subdoligranulum
uncultured bacterium

Revision editor(s): Andre, Peace Sandy, Folakunmi

Signature 2

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Reviewed Marked as Reviewed by Folakunmi on 2024-1-8

Curated date: 2023/09/24

Curator: Andre

Revision editor(s): Andre, Peace Sandy, Folakunmi

Source: Fig 4

Description: Linear discriminant analysis effect size (LEfSe) analysis of microbial abundance in the vaginal sample between adenomyosis and normal uterus.

Abundance in Group 1: decreased abundance in adenomyosis patients

NCBI	Quality Control	Links
Clostridia
Fastidiosipila
Megasphaera
Oscillospiraceae
uncultured bacterium

Revision editor(s): Andre, Peace Sandy, Folakunmi