Comparison of the ocular microbiome between chronic Stevens-Johnson syndrome patients and healthy subjects

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2023-12-30

study design

case-control

Citation

PMID PubMed identifier for scientific articles.

32152391

DOI Digital object identifier for electronic documents.

https://doi.org/10.1038/s41598-020-60794-w

URI

Authors

Kittipibul T, Puangsricharern V, Chatsuwan T

Journal

Scientific reports

Year

2020

Stevens - Johnson syndrome (SJS) has manifestation through the exfoliation of epidermis and mucosal tissue. Ocular surface is usually affected in acute and chronic stage. The patients are usually suffered from chronic ocular sequelae including symblepharon, limbal stem cell deficiency, etc. Furthermore, ocular microbiome may also be altered in SJS. This is prospective, age and sex matched analytical study which including 20 chronic SJS patients and 20 healthy subjects for specimen collection from inferior conjunctiva for microbiome analysis by conventional cultures and Next-Generation Sequencing (NGS) methods. Significant higher proportion of positive-cultured specimen was demonstrated in SJS group (SJS group 60%, healthy 10%, p-value = 0.001). In addition, NGS which providing high-throughput sequencing has demonstrated the greater diversity of microbial species. The higher proportion of pathogenic microorganisms including Pseudomonas spp., Staphylococcus spp., Streptococcus spp., Acinetobacter spp. was shown in SJS group. Ocular surface in SJS is usually occupied by more diverse microorganisms with increased proportion of pathogenic species. This condition may affect chronic inflammation and opportunistic infections in SJS group. In order to prevent and treat infection in these patients, appropriate antibiotics based on bacterial examination should be considered as the first-line treatment in the SJS patients.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2023-12-30

Curated date: 2023/09/24

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, Peace Sandy, WikiWorks

Subjects

Location of subjects: Thailand

Host species Species from which microbiome was sampled. Contact us to have more species added.: Homo sapiens

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Conjunctival sac

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Stevens-Johnson syndrome Dermatostomatitis, Stevens Johnson type,erythema multiforme Major,hypersensitivity syndrome, carbamazepine-induced, susceptibility to,severe cutaneous adverse reaction, susceptibility to,Stevens Johnson syndrome,Stevens-Johnson syndrome,Stevens-Johnson syndrome, susceptibility to,toxic Epidermal necrolysis, susceptibility to,stevens-Johnson syndrome

Group 0 name Corresponds to the control (unexposed) group for case-control studies: healthy controls

Group 1 name Corresponds to the case (exposed) group for case-control studies: Stevens-Johnson Syndrome

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Stevens-Johnson Syndrome patients

Group 0 sample size Number of subjects in the control (unexposed) group: 20

Group 1 sample size Number of subjects in the case (exposed) group: 20

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): Topical antibiotics within the 4 weeks prior to study entry were excluded

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V3-V4

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Illumina

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: relative abundances

Statistical test: Mann-Whitney (Wilcoxon)

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

Matched on Factors on which subjects have been matched on in a case-control study: age, sex

Alpha Diversity

Shannon Estimator of species richness and species evenness: more weight on species richness: increased

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2023-12-30

Curated date: 2023/09/24

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, WikiWorks

Source: Figure 1

Description: Figure 1. Significant difference of taxa abundance. This figure represents the significant difference of taxonomic level of each genus comparing between SJS patients and healthy subjects. We analyzed the proportion of each genus by Mann-Whitney U test. The p-value less than 0.05 was defined as statistically significant difference.

Abundance in Group 1: increased abundance in Stevens-Johnson Syndrome

NCBI	Quality Control	Links
Bacteroides
Faecalibacterium
Salinivibrio
Akkermansia
Prevotella
Coprococcus
Streptococcus
Lactobacillus
Fusobacterium
Bifidobacterium
Blautia
Bacillus
Phascolarctobacterium
Paraprevotella
Acinetobacter
Ruminococcus
Megamonas
Odoribacter
Staphylococcus
Pseudoalteromonas
Erwinia
Pseudomonas
Collinsella
Methanobrevibacter
Veillonella
Thermomonas
Roseburia
Turicibacter

Revision editor(s): Mary Bearkland, WikiWorks

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2023-12-30

Curated date: 2023/09/24

Curator: Mary Bearkland

Revision editor(s): Mary Bearkland, WikiWorks

Source: Figure 1

Description: Figure 1. Significant difference of taxa abundance. This figure represents the significant difference of taxonomic level of each genus comparing between SJS patients and healthy subjects. We analyzed the proportion of each genus by Mann-Whitney U test. The p-value less than 0.05 was defined as statistically significant difference.

Abundance in Group 1: decreased abundance in Stevens-Johnson Syndrome

NCBI	Quality Control	Links
Shewanella
Cohaesibacter
Clostridium
Fusibacter
Tenacibaculum
Methylophaga
Cetobacterium
Arcobacter
Vibrio

Revision editor(s): Mary Bearkland, WikiWorks