Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya

Study information

Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20

study design

time series / longitudinal observational

Citation

PMID PubMed identifier for scientific articles.

21957459

DOI Digital object identifier for electronic documents.

10.1371/journal.pone.0024767

URI

Authors

Wang Y, Gilbreath TM, Kukutla P, Yan G, Xu J

Journal

PloS one

Year

2011

The mosquito gut represents an ecosystem that accommodates a complex, intimately associated microbiome. It is increasingly clear that the gut microbiome influences a wide variety of host traits, such as fitness and immunity. Understanding the microbial community structure and its dynamics across mosquito life is a prerequisite for comprehending the symbiotic relationship between the mosquito and its gut microbial residents. Here we characterized gut bacterial communities across larvae, pupae and adults of Anopheles gambiae reared in semi-natural habitats in Kenya by pyrosequencing bacterial 16S rRNA fragments. Immatures and adults showed distinctive gut community structures. Photosynthetic Cyanobacteria were predominant in the larval and pupal guts while Proteobacteria and Bacteroidetes dominated the adult guts, with core taxa of Enterobacteriaceae and Flavobacteriaceae. At the adult stage, diet regime (sugar meal and blood meal) significantly affects the microbial structure. Intriguingly, blood meals drastically reduced the community diversity and favored enteric bacteria. Comparative genomic analysis revealed that the enriched enteric bacteria possess large genetic redox capacity of coping with oxidative and nitrosative stresses that are associated with the catabolism of blood meal, suggesting a beneficial role in maintaining gut redox homeostasis. Interestingly, gut community structure was similar in the adult stage between the field and laboratory mosquitoes, indicating that mosquito gut is a selective eco-environment for its microbiome. This comprehensive gut metatgenomic profile suggests a concerted symbiotic genetic association between gut inhabitants and host.

Experiment 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20

Curated date: 2023/10/03

Curator: Jxunmsu

Revision editor(s): Jxunmsu, LGeistlinger, Peace Sandy

Subjects

Location of subjects: Kenya

Host species Species from which microbiome was sampled. Contact us to have more species added.: Anopheles gambiae

Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology: Instar larval stage , Pupa Instar larval stage,instar larval stage,Aurelia,Chrysalides,Chrysalis,Pupae,Pupa,pupa

Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology: Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Pupal (Triplicates)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Newly emerged adult guts

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: 1-day-old adult, no feeding (triplicates)

Group 0 sample size Number of subjects in the control (unexposed) group: 9

Group 1 sample size Number of subjects in the case (exposed) group: 6

Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any): NIL

Lab analysis

Sequencing type: 16S

16S variable region One or more hypervariable region(s) of the bacterial 16S gene: V1-V3

Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance: Roche454

Statistical Analysis

Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).: raw counts

Statistical test: Metastats

Significance threshold p-value or FDR threshold used for differential abundance testing (if any): 0.05

MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?: No

Alpha Diversity

Chao1 Abundance-based estimator of species richness: decreased

Richness Number of species: decreased

Signature 1

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20

Curated date: 2024/02/20

Curator: Peace Sandy

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Source: Table S5

Description: Differentially abundant families between pupal and newly emerged adult guts

The differential abundant taxa between collections were detected with Metastats

Abundance in Group 1: increased abundance in Newly emerged adult guts

NCBI	Quality Control	Links
Enterobacteriaceae
Propionibacteriaceae

Revision editor(s): Peace Sandy

Signature 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20

Curated date: 2024/02/20

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Source: Table S5

Description: Differentially abundant families between pupal and newly emerged adult guts

The differential abundant taxa between collections were detected with Metastats

Abundance in Group 1: increased abundance in Newly emerged adult guts

NCBI	Quality Control	Links
Comamonadaceae
Erythrobacteraceae
Paracoccaceae
Aeromonadaceae
Rhodanobacteraceae

Revision editor(s): Peace Sandy

Experiment 2

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Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20

Curated date: 2024/02/20

Curator: Peace Sandy

Revision editor(s): Peace Sandy

Differences from previous experiment shown

Subjects

Group 0 name Corresponds to the control (unexposed) group for case-control studies: Sugar fed mosquito - 3-day-old,sugar (triplicates)

Group 1 name Corresponds to the case (exposed) group for case-control studies: Blood fed mosquito - 2 days post blood meal (triplicates)

Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group: Blood-fed mosquito at different time points