Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya/Experiment 1
From BugSigDB
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Subjects
- Location of subjects
- Kenya
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Anopheles gambiae
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Instar larval stage , Pupa Instar larval stage,instar larval stage,Aurelia,Chrysalides,Chrysalis,Pupae,Pupa,pupa
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Gut microbiome measurement Gut microbiome measurement,gut microbiome measurement
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Pupal (Triplicates)
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Newly emerged adult guts
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- 1-day-old adult, no feeding (triplicates)
- Group 0 sample size Number of subjects in the control (unexposed) group
- 9
- Group 1 sample size Number of subjects in the case (exposed) group
- 6
- Antibiotics exclusion Number of days without antibiotics usage (if applicable) and other antibiotics-related criteria used to exclude participants (if any)
- NIL
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V1-V3
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Roche454
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- raw counts
- Statistical test
- Metastats
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Source: Table S5
Description: Differentially abundant families between pupal and newly emerged adult guts
The differential abundant taxa between collections were detected with Metastats
Abundance in Group 1: increased abundance in Newly emerged adult guts
| NCBI | Quality Control | Links |
|---|---|---|
| Enterobacteriaceae | ||
| Propionibacteriaceae |
Revision editor(s): Peace Sandy
Signature 2
Reviewed Marked as Reviewed by Peace Sandy on 2024-2-20
Source: Table S5
Description: Differentially abundant families between pupal and newly emerged adult guts
The differential abundant taxa between collections were detected with Metastats
Abundance in Group 1: increased abundance in Newly emerged adult guts
| NCBI | Quality Control | Links |
|---|---|---|
| Comamonadaceae | ||
| Erythrobacteraceae | ||
| Paracoccaceae | ||
| Aeromonadaceae | ||
| Rhodanobacteraceae |
Revision editor(s): Peace Sandy
