Urinary Microbiome and Psychological Factors in Women with Overactive Bladder
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Study information
-
Quality control
- Retracted paper
- Contamination issues suspected
- Batch effect issues suspected
- Uncontrolled confounding suspected
- Results are suspect (various reasons)
- Tags applied
study design
Citation
PMID PubMed identifier for scientific articles.
DOI Digital object identifier for electronic documents.
Authors
Wu P, Chen Y, Zhao J, Zhang G, Chen J, Wang J, Zhang H
Journal
Frontiers in cellular and infection microbiology
Year
2017
Keywords:
bacteria, depression, overactive bladder, psychology, urinary microbiome
Objectives: Emerging evidence indicates that alterations to the urinary microbiome are related to lower urinary tract symptoms. Overactive bladder (OAB) is a common disorder with complex etiologies and usually accompanied by psychological diseases. More information concerning the urinary microbiome and psychological factors in OAB is required. The aim of this study was to characterize the female urinary microbiome associated with OAB and investigate the relationships between urinary microbiome and psychological factors. Methods: Thirty women with OAB and 25 asymptomatic controls were recruited and asked to finish the Overactive Bladder Symptom Score, Self-Rating Anxiety Scale and Self-Rating Depression Scale. Urine specimens were collected by transurethral catheterization and processed for 16S rRNA gene sequencing using Illumina MiSeq. Sequencing reads were processed using QIIME. LEfSe revealed significant differences in bacterial genera between controls and OAB patients. The relationships between the diversity of the urinary microbiome and psychological scores were identified by Pearson's correlation coefficient. Results: We found that bacterial diversity (Simpson index) and richness (Chao1) were lower in OAB samples compared to controls (P both = 0.038). OAB and control bacterial communities were significantly different (based on weighted UniFrac distance metric, R = 0.064, P = 0.037). LEfSe demonstrated that 7 genera were increased (e.g., Proteus and Aerococcus) and 13 were reduced (e.g., Lactobacillus and Prevotella) in OAB group compared to controls. There were negative correlations between scores on Self-Rating Depression Scale and both richness (Chao1, r = -0.458, P = 0.011) and diversity (Shannon index, r = -0.516, P = 0.003) of urinary microbiome in OAB group. Some bacterial genera of OAB women with anxiety or depression were significantly different from those without. Conclusions: The aberrant urinary microbiome with decreased diversity and richness may have strong implications in pathogenesis and treatment of OAB. Psychological conditions were correlated with characteristics of urinary microbiome in women with OAB. Further research is needed to understand the connection between central nervous system and urinary microbiome.
Experiment 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Subjects
- Location of subjects
- China
- Host species Species from which microbiome was sampled. Contact us to have more species added.
- Homo sapiens
- Body site Anatomical site where microbial samples were extracted from according to the Uber Anatomy Ontology
- Female urethra Urethra feminina,Urethra muliebris,Female urethra,female urethra
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Overactive bladder Hypertonic bladder,overactive bladder,overactive bladder (disease),Overactive Detrusor,Urinary Bladder Overactive,Overactive bladder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Asymptomatic controls
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Overactive Bladder (OAB) patients
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Adult patients aged 18 or above, diagnosed with OAB
- Group 0 sample size Number of subjects in the control (unexposed) group
- 25
- Group 1 sample size Number of subjects in the case (exposed) group
- 30
Lab analysis
- Sequencing type
- 16S
- 16S variable region One or more hypervariable region(s) of the bacterial 16S gene
- V4
- Sequencing platform Manufacturer and experimental platform used for quantifying microbial abundance
- Illumina
Statistical Analysis
- Data transformation Data transformation applied to microbial abundance measurements prior to differential abundance testing (if any).
- relative abundances
- Statistical test
- LEfSe
- Significance threshold p-value or FDR threshold used for differential abundance testing (if any)
- 0.05
- MHT correction Have statistical tests be corrected for multiple hypothesis testing (MHT)?
- No
- LDA Score above Threshold for the linear discriminant analysis (LDA) score for studies using the popular LEfSe tool
- 2.5
Alpha Diversity
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Source: Figure 4
Description: Urinary microbiomes that were significantly more increased in the samples of female patients with OAB (Over Active Bladder) compared to the control samples from asymptomatic women
Abundance in Group 1: increased abundance in Overactive Bladder (OAB) patients
| NCBI | Quality Control | Links |
|---|---|---|
| Staphylococcus | ||
| Sneathia | ||
| Proteus | ||
| Helcococcus | ||
| Gemella | ||
| Mycoplasma | ||
| Aerococcus |
Revision editor(s): Ojotuleonalo
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Source: Figure 4
Description: Urinary microbiomes that were significantly more decreased in the samples of female patients with OAB (Over Active Bladder) compared to the control samples from asymptomatic women
Abundance in Group 1: decreased abundance in Overactive Bladder (OAB) patients
| NCBI | Quality Control | Links |
|---|---|---|
| Anaerococcus | ||
| Campylobacter | ||
| Dialister | ||
| Enterococcus | ||
| Finegoldia | ||
| Fusobacterium | ||
| Jonquetella | ||
| Lactobacillus | ||
| Lactococcus | ||
| Novosphingobium | ||
| Prevotella | ||
| Pyramidobacter | ||
| Ureaplasma |
Revision editor(s): Ojotuleonalo
Experiment 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Anxiety Anxiety,anxiety
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Overactive Bladder (OAB) without anxiety
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Overactive Bladder (OAB) with anxiety
- Group 1 definition Diagnostic criteria applied to define the specific condition / phenotype represented in the case (exposed) group
- Adult patients aged 18 or above, diagnosed with OAB and have anxiety
- Group 0 sample size Number of subjects in the control (unexposed) group
- 18
- Group 1 sample size Number of subjects in the case (exposed) group
- 12
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- increased
- Chao1 Abundance-based estimator of species richness
- increased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- increased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Curated date: 2023/10/08
Curator: Ojotuleonalo
Revision editor(s): Ojotuleonalo, Peace Sandy, Folakunmi
Source: Figure 5C
Description: Urinary microbiomes that were significantly more increased in the samples of female patients with OAB (Over Active Bladder) with anxiety compared to the samples of female patients with OAB without anxiety
Abundance in Group 1: increased abundance in Overactive Bladder (OAB) with anxiety
| NCBI | Quality Control | Links |
|---|---|---|
| Actinobaculum | ||
| Alicyclobacillus | ||
| Gallicola | ||
| Helcococcus | ||
| Leptolyngbya | ||
| Porphyromonas | ||
| Sneathia |
Revision editor(s): Ojotuleonalo, Peace Sandy, Folakunmi
Signature 2
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Curated date: 2023/10/08
Curator: Ojotuleonalo
Revision editor(s): Ojotuleonalo, Peace Sandy, Folakunmi
Source: Figure 5C
Description: Urinary microbiomes that were significantly more decreased in the samples of female patients with OAB (Over Active Bladder) with anxiety compared to the samples of female patients with OAB without anxiety.
Abundance in Group 1: decreased abundance in Overactive Bladder (OAB) with anxiety
| NCBI | Quality Control | Links |
|---|---|---|
| Agrobacterium | ||
| Burkholderia | ||
| Collinsella | ||
| Cryocola | ||
| Methanobrevibacter | ||
| Psychrobacter |
Revision editor(s): Ojotuleonalo, Peace Sandy, Folakunmi
Experiment 3
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Differences from previous experiment shown
Subjects
- Condition The experimental condition / phenotype studied according to the Experimental Factor Ontology
- Depressive disorder depression,melancholia,melancholias,mental depression,syndrome, depressive,syndromes, depressive,Depressive disorder,depressive disorder
- Group 0 name Corresponds to the control (unexposed) group for case-control studies
- Overactive Bladder (OAB) without depression
- Group 1 name Corresponds to the case (exposed) group for case-control studies
- Overactive Bladder (OAB) with depression
- Group 0 sample size Number of subjects in the control (unexposed) group
- 16
- Group 1 sample size Number of subjects in the case (exposed) group
- 14
Lab analysis
Statistical Analysis
Alpha Diversity
- Pielou Quantifies how equal the community is numerically
- increased
- Shannon Estimator of species richness and species evenness: more weight on species richness
- decreased
- Chao1 Abundance-based estimator of species richness
- decreased
- Simpson Estimator of species richness and species evenness: more weight on species evenness
- decreased
- Richness Number of species
- decreased
Signature 1
Reviewed Marked as Reviewed by Folakunmi on 2024-1-18
Source: Figure 5D
Description: Urinary microbiomes that were significantly decreased in the samples of female patients with OAB (Over Active Bladder) with depression compared to the samples of female patients with OAB without depression
Abundance in Group 1: decreased abundance in Overactive Bladder (OAB) with depression
| NCBI | Quality Control | Links |
|---|---|---|
| Clostridium | ||
| Actinobaculum | ||
| Pedobacter | ||
| Sporobacterium sp. WAL 1855D |
Revision editor(s): Folakunmi
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